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6de6
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6de6 is ON HOLD until Paper Publication Authors: Goyal, P., Lovell, S., Richter, M. Description: 2.1 A resolution structure of histamine dehydrogen...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==2.1 A resolution structure of histamine dehydrogenase from Rhizobium sp. 4-9== | |
| + | <StructureSection load='6de6' size='340' side='right'caption='[[6de6]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6de6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhizobium_sp._4-9 Rhizobium sp. 4-9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DE6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DE6 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6de6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6de6 OCA], [https://pdbe.org/6de6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6de6 RCSB], [https://www.ebi.ac.uk/pdbsum/6de6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6de6 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q60I59_9HYPH Q60I59_9HYPH] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Histamine dehydrogenase from the gram-negative bacterium Rhizobium sp. 4-9 (HaDHR) is a member of a small family of dehydrogenases containing a covalently attached FMN, and the only member so far identified to date that does not exhibit substrate inhibition. In this study, we present the 2.1 A resolution crystal structure of HaDHR. This new structure allowed for the identification of the internal electron transfer pathway to abiological ferrocene-based mediators. Alanine 437 was identified as the exit point of electrons from the Fe(4)S(4) cluster. The enzyme was modified with a Ser436Cys mutation to facilitate covalent attachment of a ferrocene moiety. When modified with Fc-maleimide, this new construct demonstrated direct electron transfer from the enzyme to a gold electrode in a histamine concentration-dependent manner without the need for any additional electron mediators. | ||
| - | + | Structure of Rhizobium sp. 4-9 histamine dehydrogenase and analysis of the electron transfer pathway to an abiological electron acceptor.,Goyal P, Deay D 3rd, Seibold S, Candido ACL, Lovell S, Battaile KP, Wilson GS, Richter ML, Petillo PA Arch Biochem Biophys. 2023 May 3;742:109612. doi: 10.1016/j.abb.2023.109612. PMID:37146865<ref>PMID:37146865</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6de6" style="background-color:#fffaf0;"></div> |
| - | [[Category: Goyal | + | == References == |
| - | [[Category: Richter | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Rhizobium sp. 4-9]] | ||
| + | [[Category: Goyal P]] | ||
| + | [[Category: Lovell S]] | ||
| + | [[Category: Richter M]] | ||
Current revision
2.1 A resolution structure of histamine dehydrogenase from Rhizobium sp. 4-9
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