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| - | [[Image:2ief.gif|left|200px]] | |
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| - | {{Structure
| + | ==Structure of the cooperative Excisionase (Xis)-DNA complex reveals a micronucleoprotein filament== |
| - | |PDB= 2ief |SIZE=350|CAPTION= <scene name='initialview01'>2ief</scene>, resolution 2.601Å
| + | <StructureSection load='2ief' size='340' side='right'caption='[[2ief]], [[Resolution|resolution]] 2.60Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>
| + | <table><tr><td colspan='2'>[[2ief]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IEF FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.601Å</td></tr> |
| - | |GENE= Xis ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Enterobacteria phage lambda])
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ief FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ief OCA], [https://pdbe.org/2ief PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ief RCSB], [https://www.ebi.ac.uk/pdbsum/2ief PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ief ProSAT]</span></td></tr> |
| - | |DOMAIN=
| + | </table> |
| - | |RELATEDENTRY=
| + | == Function == |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ief FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ief OCA], [http://www.ebi.ac.uk/pdbsum/2ief PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ief RCSB]</span>
| + | [https://www.uniprot.org/uniprot/VXIS_LAMBD VXIS_LAMBD] Excisionase and integrase are necessary for the excision of prophage from the host genome by site-specific recombination at the att site. |
| - | }}
| + | __TOC__ |
| - | | + | </StructureSection> |
| - | '''Structure of the cooperative Excisionase (Xis)-DNA complex reveals a micronucleoprotein filament'''
| + | [[Category: Escherichia virus Lambda]] |
| - | | + | [[Category: Large Structures]] |
| - | | + | [[Category: Abbani MA]] |
| - | ==Overview==
| + | [[Category: Cascio D]] |
| - | The DNA architectural protein Xis regulates the construction of higher-order nucleoprotein intasomes that integrate and excise the genome of phage lambda from the Escherichia coli chromosome. Xis modulates the directionality of site-specific recombination by stimulating phage excision 10(6)-fold, while simultaneously inhibiting phage reintegration. Control is exerted by cooperatively assembling onto a approximately 35-bp DNA regulatory element, which it distorts to preferentially stabilize an excisive intasome. Here, we report the 2.6-A crystal structure of the complex between three cooperatively bound Xis proteins and a 33-bp DNA containing the regulatory element. Xis binds DNA in a head-to-tail orientation to generate a micronucleoprotein filament. Although each protomer is anchored to the duplex by a similar set of nonbase specific contacts, malleable protein-DNA interactions enable binding to sites that differ in nucleotide sequence. Proteins at the ends of the duplex sequence specifically recognize similar binding sites and participate in cooperative binding via protein-protein interactions with a bridging Xis protomer that is bound in a less specific manner. Formation of this polymer introduces approximately 72 degrees of curvature into the DNA with slight positive writhe, which functions to connect disparate segments of DNA bridged by integrase within the excisive intasome.
| + | [[Category: Clubb RT]] |
| - | | + | [[Category: Johnson RC]] |
| - | ==About this Structure==
| + | [[Category: Papagiannis CV]] |
| - | 2IEF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IEF OCA].
| + | [[Category: Sam MD]] |
| - | | + | |
| - | ==Reference== | + | |
| - | Structure of the cooperative Xis-DNA complex reveals a micronucleoprotein filament that regulates phage lambda intasome assembly., Abbani MA, Papagiannis CV, Sam MD, Cascio D, Johnson RC, Clubb RT, Proc Natl Acad Sci U S A. 2007 Feb 13;104(7):2109-14. Epub 2007 Feb 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17287355 17287355]
| + | |
| - | [[Category: Enterobacteria phage lambda]] | + | |
| - | [[Category: Single protein]] | + | |
| - | [[Category: Abbani, M A.]] | + | |
| - | [[Category: Cascio, D.]] | + | |
| - | [[Category: Clubb, R T.]] | + | |
| - | [[Category: Johnson, R C.]] | + | |
| - | [[Category: Papagiannis, C V.]] | + | |
| - | [[Category: Sam, M D.]] | + | |
| - | [[Category: excisionase]]
| + | |
| - | [[Category: micronucleoprotein filament]]
| + | |
| - | [[Category: recombination directionality factor]]
| + | |
| - | [[Category: site specific recombination]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:43:11 2008''
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