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| | ==Structural Basis for Katanin Self-Assembly== | | ==Structural Basis for Katanin Self-Assembly== |
| - | <StructureSection load='6b5c' size='340' side='right' caption='[[6b5c]], [[Resolution|resolution]] 2.40Å' scene=''> | + | <StructureSection load='6b5c' size='340' side='right'caption='[[6b5c]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6b5c]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B5C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B5C FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6b5c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B5C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6B5C FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Microtubule-severing_ATPase Microtubule-severing ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.3 3.6.4.3] </span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b5c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b5c OCA], [http://pdbe.org/6b5c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b5c RCSB], [http://www.ebi.ac.uk/pdbsum/6b5c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b5c ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6b5c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b5c OCA], [https://pdbe.org/6b5c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6b5c RCSB], [https://www.ebi.ac.uk/pdbsum/6b5c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6b5c ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/KATL1_HUMAN KATL1_HUMAN]] Regulates microtubule dynamics in Sertoli cells, a process that is essential for spermiogenesis and male fertility. Severs microtubules in an ATP-dependent manner, promoting rapid reorganization of cellular microtubule arrays (By similarity). Has microtubule-severing activity in vitro (PubMed:26929214).[UniProtKB:Q8K0T4]<ref>PMID:26929214</ref> | + | [https://www.uniprot.org/uniprot/KATL1_HUMAN KATL1_HUMAN] Regulates microtubule dynamics in Sertoli cells, a process that is essential for spermiogenesis and male fertility. Severs microtubules in an ATP-dependent manner, promoting rapid reorganization of cellular microtubule arrays (By similarity). Has microtubule-severing activity in vitro (PubMed:26929214).[UniProtKB:Q8K0T4]<ref>PMID:26929214</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Microtubule-severing ATPase]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Al-Bassam, J]] | + | [[Category: Large Structures]] |
| - | [[Category: Nithianantham, S]] | + | [[Category: Al-Bassam J]] |
| - | [[Category: Aaa atpase]] | + | [[Category: Nithianantham S]] |
| - | [[Category: Cell cycle]]
| + | |
| - | [[Category: Katanin]]
| + | |
| - | [[Category: Microtubule severing protein]]
| + | |
| Structural highlights
Function
KATL1_HUMAN Regulates microtubule dynamics in Sertoli cells, a process that is essential for spermiogenesis and male fertility. Severs microtubules in an ATP-dependent manner, promoting rapid reorganization of cellular microtubule arrays (By similarity). Has microtubule-severing activity in vitro (PubMed:26929214).[UniProtKB:Q8K0T4][1]
Publication Abstract from PubMed
The reorganization of microtubules in mitosis, meiosis and development requires the microtubule-severing activity of katanin. Katanin is a heterodimer composed of an ATPase Associated with diverse cellular Activities (AAA) subunit and a regulatory subunit. Microtubule severing requires ATP hydrolysis by katanin's conserved AAA ATPase domains. Whereas other AAA ATPases form stable hexamers, we show that katanin only forms monomer or dimers of heterodimers in solution. Katanin oligomers consistent with hexamers of heterodimers or heterododecamers were only observed for an ATP hydrolysis deficient mutant in the presence of ATP. X-ray structures of katanin's AAA ATPase in monomeric nucleotide-free and pseudo-oligomeric ADP-bound states reveal conformational changes in AAA subdomains that explained the structural basis for instability of katanin heterododecamer. We propose that the rapid dissociation of katanin AAA oligomers may lead to an auto-inhibited state that prevents inappropriate microtubule severing, or that cyclical disassembly into heterodimers may critically contribute to the microtubule-severing mechanism.
Structural Basis for Disassembly of Katanin Heterododecamers.,Nithianantham S, McNally FJ, Al-Bassam J J Biol Chem. 2018 May 11. pii: RA117.001215. doi: 10.1074/jbc.RA117.001215. PMID:29752405[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Cheung K, Senese S, Kuang J, Bui N, Ongpipattanakul C, Gholkar A, Cohn W, Capri J, Whitelegge JP, Torres JZ. Proteomic Analysis of the Mammalian Katanin Family of Microtubule-severing Enzymes Defines Katanin p80 subunit B-like 1 (KATNBL1) as a Regulator of Mammalian Katanin Microtubule-severing. Mol Cell Proteomics. 2016 May;15(5):1658-69. doi: 10.1074/mcp.M115.056465. Epub, 2016 Feb 29. PMID:26929214 doi:http://dx.doi.org/10.1074/mcp.M115.056465
- ↑ Nithianantham S, McNally FJ, Al-Bassam J. Structural Basis for Disassembly of Katanin Heterododecamers. J Biol Chem. 2018 May 11. pii: RA117.001215. doi: 10.1074/jbc.RA117.001215. PMID:29752405 doi:http://dx.doi.org/10.1074/jbc.RA117.001215
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