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| | ==Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface== | | ==Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface== |
| - | <StructureSection load='6cfz' size='340' side='right' caption='[[6cfz]], [[Resolution|resolution]] 4.50Å' scene=''> | + | <SX load='6cfz' size='340' side='right' viewer='molstar' caption='[[6cfz]], [[Resolution|resolution]] 4.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6cfz]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Cbs_144.50 Cbs 144.50] and [http://en.wikipedia.org/wiki/Chatd Chatd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CFZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6cfz]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum Chaetomium thermophilum] and [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CFZ FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CTHT_0029740 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=209285 CBS 144.50]), CTHT_0016520 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD]), CTHT_0005680 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=209285 CBS 144.50]), CTHT_0002350 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=209285 CBS 144.50]), CTHT_0042930 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=209285 CBS 144.50]), CTHT_0032660 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=209285 CBS 144.50]), CTHT_0006450 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=209285 CBS 144.50]), CTHT_0017540 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=209285 CBS 144.50]), CTHT_0010600 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=209285 CBS 144.50])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.5Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cfz OCA], [http://pdbe.org/6cfz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cfz RCSB], [http://www.ebi.ac.uk/pdbsum/6cfz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cfz ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cfz OCA], [https://pdbe.org/6cfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cfz RCSB], [https://www.ebi.ac.uk/pdbsum/6cfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cfz ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/G0S8F9_CHATD G0S8F9_CHATD] |
| - | Kinetochores connect mitotic-spindle microtubules with chromosomes, allowing microtubule depolymerization to pull chromosomes apart during anaphase while resisting detachment as the microtubule shortens. The heterodecameric DASH/Dam1 complex (DASH/Dam1c), an essential component of yeast kinetochores, assembles into a microtubule-encircling ring. The ring associates with rodlike Ndc80 complexes to organize the kinetochore-microtubule interface. We report the cryo-electron microscopy structure (at ~4.5-angstrom resolution) of a DASH/Dam1c ring and a molecular model of its ordered components, validated by evolutionary direct-coupling analysis. Integrating this structure with that of the Ndc80 complex and with published interaction data yields a molecular picture of kinetochore-microtubule attachment, including how flexible, C-terminal extensions of DASH/Dam1c subunits project and contact widely separated sites on the Ndc80 complex rod and how phosphorylation at previously identified sites might regulate kinetochore assembly.
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| - | Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface.,Jenni S, Harrison SC Science. 2018 May 4;360(6388):552-558. doi: 10.1126/science.aar6436. PMID:29724956<ref>PMID:29724956</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 6cfz" style="background-color:#fffaf0;"></div>
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| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| - | </StructureSection> | + | </SX> |
| - | [[Category: Cbs 144 50]] | + | [[Category: Chaetomium thermophilum]] |
| - | [[Category: Chatd]] | + | [[Category: Chaetomium thermophilum var. thermophilum DSM 1495]] |
| - | [[Category: Harrison, S C]] | + | [[Category: Large Structures]] |
| - | [[Category: Jenni, S]] | + | [[Category: Harrison SC]] |
| - | [[Category: Ask1]] | + | [[Category: Jenni S]] |
| - | [[Category: Cell-division]]
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| - | [[Category: Chromosome segregation]]
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| - | [[Category: Dad1]]
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| - | [[Category: Dad2]]
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| - | [[Category: Dad3]]
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| - | [[Category: Dad4]]
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| - | [[Category: Dam1]]
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| - | [[Category: Dam1 complex]]
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| - | [[Category: Dash]]
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| - | [[Category: Duo1]]
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| - | [[Category: Hsk3]]
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| - | [[Category: Kinetochore]]
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| - | [[Category: Kinetochore-microtubule interface]]
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| - | [[Category: Nuclear protein]]
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| - | [[Category: Point-centromere yeast]]
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| - | [[Category: Spc19]]
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| - | [[Category: Spc34]]
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