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| | ==ENAIIHis Fab fragment in the free form== | | ==ENAIIHis Fab fragment in the free form== |
| - | <StructureSection load='2c1o' size='340' side='right' caption='[[2c1o]], [[Resolution|resolution]] 2.75Å' scene=''> | + | <StructureSection load='2c1o' size='340' side='right'caption='[[2c1o]], [[Resolution|resolution]] 2.75Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2c1o]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C1O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2C1O FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2c1o]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C1O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2C1O FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1afv|1afv]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c1o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c1o OCA], [http://pdbe.org/2c1o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2c1o RCSB], [http://www.ebi.ac.uk/pdbsum/2c1o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2c1o ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2c1o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c1o OCA], [https://pdbe.org/2c1o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2c1o RCSB], [https://www.ebi.ac.uk/pdbsum/2c1o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2c1o ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q58EU8_MOUSE Q58EU8_MOUSE] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Koivula, A]] | + | [[Category: Mus musculus]] |
| - | [[Category: Nevanen, T K]] | + | [[Category: Koivula A]] |
| - | [[Category: Parkkinen, T]] | + | [[Category: Nevanen TK]] |
| - | [[Category: Rouvinen, J]] | + | [[Category: Parkkinen T]] |
| - | [[Category: Antibody]]
| + | [[Category: Rouvinen J]] |
| - | [[Category: Ena11his antibody]]
| + | |
| - | [[Category: Enantioselective]]
| + | |
| - | [[Category: Fab fragment]]
| + | |
| - | [[Category: Finrozole]]
| + | |
| - | [[Category: Immune system]]
| + | |
| - | [[Category: Immunoglobulin domain]]
| + | |
| Structural highlights
Function
Q58EU8_MOUSE
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Enantioselective antibodies can separate the enantiomers of a chiral compound in a highly specific manner. We have recently reported the cloning and applications of a recombinant Fab-fragment, ENA11His, in the enantioseparation of a drug candidate, finrozole, which contains two chiral centers. Here, the crystal structures of this enantioselective antibody Fab-fragment are determined in the absence of the hapten at a resolution of 2.75 A, and in the presence of the hapten at 2.05 A resolution. The conformation of the protein was found to be similar in both free and complex forms. The hapten molecule was tightly bound in a deep cleft between the light and heavy chains of the Fab-fragment. The complex structure also allowed us to describe the molecular basis for enantioselectivity and to deduce the absolute configurations of all the four different stereoisomers (a-d) of finrozole. The ENA11His antibody fragment selectively binds the SR (a) enantiomer from the racemic mixture of a and d-enantiomers, thus allowing separation from the pharmacologically most active RS enantiomer (d). In particular, Asp95 and Asn35 of the H-chain in the ENA11 His antibody seem to provide this specificity through hydrogen bonding.
Crystal structures of an enantioselective fab-fragment in free and complex forms.,Parkkinen T, Nevanen TK, Koivula A, Rouvinen J J Mol Biol. 2006 Mar 24;357(2):471-80. Epub 2006 Jan 3. PMID:16427081[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Parkkinen T, Nevanen TK, Koivula A, Rouvinen J. Crystal structures of an enantioselective fab-fragment in free and complex forms. J Mol Biol. 2006 Mar 24;357(2):471-80. Epub 2006 Jan 3. PMID:16427081 doi:10.1016/j.jmb.2005.12.045
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