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| | ==Structure of the IMS domain of the mitochondrial import protein Tim21 from S. cerevisiae== | | ==Structure of the IMS domain of the mitochondrial import protein Tim21 from S. cerevisiae== |
| - | <StructureSection load='2ciu' size='340' side='right' caption='[[2ciu]], [[Resolution|resolution]] 1.60Å' scene=''> | + | <StructureSection load='2ciu' size='340' side='right'caption='[[2ciu]], [[Resolution|resolution]] 1.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2ciu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CIU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CIU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2ciu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CIU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CIU FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ciu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ciu OCA], [http://pdbe.org/2ciu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ciu RCSB], [http://www.ebi.ac.uk/pdbsum/2ciu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ciu ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ciu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ciu OCA], [https://pdbe.org/2ciu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ciu RCSB], [https://www.ebi.ac.uk/pdbsum/2ciu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ciu ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/TIM21_YEAST TIM21_YEAST] Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Required to keep the TOM and the TIM23 complexes in close contact. At some point, it is released from the TOM23 complex to allow protein translocation into the mitochondrial matrix. In the complex, it acts as an antagonist of TIM50 by reducing preprotein accumulation at the TOM23 complex and promotes dissociation of the PAM complex from the TIM23 complex.<ref>PMID:15797382</ref> <ref>PMID:15878866</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 18824]] | + | [[Category: Large Structures]] |
| - | [[Category: Albrecht, R]] | + | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: Pfanner, N]] | + | [[Category: Albrecht R]] |
| - | [[Category: Rehling, P]] | + | [[Category: Pfanner N]] |
| - | [[Category: Zeth, K]] | + | [[Category: Rehling P]] |
| - | [[Category: Inner membrane]]
| + | [[Category: Zeth K]] |
| - | [[Category: Membrane]]
| + | |
| - | [[Category: Mitochondrial import]]
| + | |
| - | [[Category: Mitochondrion]]
| + | |
| - | [[Category: Protein transport]]
| + | |
| - | [[Category: Transit peptide]]
| + | |
| - | [[Category: Translocation]]
| + | |
| - | [[Category: Transmembrane]]
| + | |
| - | [[Category: Transport]]
| + | |
| Structural highlights
Function
TIM21_YEAST Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Required to keep the TOM and the TIM23 complexes in close contact. At some point, it is released from the TOM23 complex to allow protein translocation into the mitochondrial matrix. In the complex, it acts as an antagonist of TIM50 by reducing preprotein accumulation at the TOM23 complex and promotes dissociation of the PAM complex from the TIM23 complex.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Proteins destined for the mitochondrial matrix are imported by the translocase of the outer membrane--the TOM complex--and the presequence translocase of the inner membrane--the TIM23 complex. At present, there is no structural information on components of the presequence translocase. Tim21, a subunit of the presequence translocase consisting of a membrane anchor and a carboxy-terminal domain exposed to the intermembrane space, directly connects the TOM and TIM23 complexes by binding to the intermembrane space domain of the Tom22 receptor. We crystallized the binding domain of Tim21 of Saccharomyces cerevisiae and determined its structure at 1.6 A resolution. The Tim21 structure represents a new alpha/beta-mixed protein fold with two alpha-helices flanked by an extended eight-stranded beta-sheet. We also identified a core sequence of Tom22 that binds to Tim21. Furthermore, negatively charged amino-acid residues of Tom22 are important for binding to Tim21. Here we suggest a mechanism for the TOM-TIM interaction.
The Tim21 binding domain connects the preprotein translocases of both mitochondrial membranes.,Albrecht R, Rehling P, Chacinska A, Brix J, Cadamuro SA, Volkmer R, Guiard B, Pfanner N, Zeth K EMBO Rep. 2006 Dec;7(12):1233-8. Epub 2006 Nov 10. PMID:17099692[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Chacinska A, Lind M, Frazier AE, Dudek J, Meisinger C, Geissler A, Sickmann A, Meyer HE, Truscott KN, Guiard B, Pfanner N, Rehling P. Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17. Cell. 2005 Mar 25;120(6):817-29. PMID:15797382 doi:http://dx.doi.org/10.1016/j.cell.2005.01.011
- ↑ Mokranjac D, Popov-Celeketic D, Hell K, Neupert W. Role of Tim21 in mitochondrial translocation contact sites. J Biol Chem. 2005 Jun 24;280(25):23437-40. Epub 2005 May 4. PMID:15878866 doi:http://dx.doi.org/C500135200
- ↑ Albrecht R, Rehling P, Chacinska A, Brix J, Cadamuro SA, Volkmer R, Guiard B, Pfanner N, Zeth K. The Tim21 binding domain connects the preprotein translocases of both mitochondrial membranes. EMBO Rep. 2006 Dec;7(12):1233-8. Epub 2006 Nov 10. PMID:17099692
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