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2cg8

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The bifunctional dihydroneopterin aldolase 6-hydroxymethyl-7,8- dihydropterin synthase from Streptococcus pneumoniae

Structural highlights

2cg8 is a 4 chain structure with sequence from Streptococcus pneumoniae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.9Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SULD_STRR6

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The enzymes dihydroneopterin aldolase (DHNA) and 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK) catalyse two consecutive steps in the biosynthesis of folic acid. Neither of these enzymes has a counterpart in mammals, and they have therefore been suggested as ideal targets for antimicrobial drugs. Some of the enzymes within the folate pathway can occur as bi- or trifunctional complexes in bacteria and parasites, but the way in which bifunctional DHNA-HPPK enzymes are assembled is unclear. Here, we report the determination of the structure at 2.9 A resolution of the DHNA-HPPK (SulD) bifunctional enzyme complex from the respiratory pathogen Streptococcus pneumoniae. In the crystal, DHNA is assembled as a core octamer, with 422 point group symmetry, although the enzyme is active as a tetramer in solution. Individual HPPK monomers are arranged at the ends of the DHNA octamer, making relatively few contacts with the DHNA domain, but more extensive interactions with adjacent HPPK domains. As a result, the structure forms an elongated cylinder, with the HPPK domains forming two tetramers at each end. The active sites of both enzymes face outward, and there is no clear channel between them that could be used for channelling substrates. The HPPK-HPPK interface accounts for about one-third of the total area between adjacent monomers in SulD, and has levels of surface complementarity comparable to that of the DHNA-DHNA interfaces. There is no "linker" polypeptide between DHNA and HPPK, reducing the conformational flexibility of the HPPK domain relative to the DHNA domain. The implications for the organisation of bi- and trifunctional enzyme complexes within the folate biosynthesis pathway are discussed.

Crystal structure of the bifunctional dihydroneopterin aldolase/6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase from Streptococcus pneumoniae.,Garcon A, Levy C, Derrick JP J Mol Biol. 2006 Jul 14;360(3):644-53. Epub 2006 May 30. PMID:16781731^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Garcon A, Levy C, Derrick JP. Crystal structure of the bifunctional dihydroneopterin aldolase/6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase from Streptococcus pneumoniae. J Mol Biol. 2006 Jul 14;360(3):644-53. Epub 2006 May 30. PMID:16781731 doi:10.1016/j.jmb.2006.05.038

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2cg8"

Categories: Large Structures | Streptococcus pneumoniae | Derrick JP | Garcon A | Levy C

@@ Line 1: / Line 1: @@
 ==The bifunctional dihydroneopterin aldolase 6-hydroxymethyl-7,8- dihydropterin synthase from Streptococcus pneumoniae==
-<StructureSection load='2cg8' size='340' side='right' caption='[[2cg8]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
+<StructureSection load='2cg8' size='340' side='right'caption='[[2cg8]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2cg8]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus pneumoniae" (klein 1884) weichselbaum 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CG8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CG8 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2cg8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CG8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CG8 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cg8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cg8 OCA], [http://pdbe.org/2cg8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cg8 RCSB], [http://www.ebi.ac.uk/pdbsum/2cg8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2cg8 ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cg8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cg8 OCA], [https://pdbe.org/2cg8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cg8 RCSB], [https://www.ebi.ac.uk/pdbsum/2cg8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cg8 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/SULD_STRR6 SULD_STRR6]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 27: / Line 30: @@
 ==See Also==
-*[[Aldolase|Aldolase]]
+*[[Aldolase 3D structures|Aldolase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Derrick, J P]]
+[[Category: Large Structures]]
-[[Category: Garcon, A]]
+[[Category: Streptococcus pneumoniae]]
-[[Category: Levy, C]]
+[[Category: Derrick JP]]
-[[Category: Aldolase]]
+[[Category: Garcon A]]
-[[Category: Folate biosynthesis]]
+[[Category: Levy C]]
-[[Category: Lyase]]
-[[Category: Lyase-transferase complex]]
-[[Category: Lyase/transferase]]
-[[Category: Multifunctional enzyme]]
-[[Category: Pyrophosphokinase]]
-[[Category: Transferase]]

2cg8

From Proteopedia

Current revision

The bifunctional dihydroneopterin aldolase 6-hydroxymethyl-7,8- dihydropterin synthase from Streptococcus pneumoniae

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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