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6gm2
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6gm2 is ON HOLD Authors: Duan, J., Esselborn, J., Hofmann, E., Winkler, M., Happe, T. Description: [FeFe]-hydrogenase CpI from Clostridium pasteuri...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==[FeFe]-hydrogenase CpI from Clostridium pasteurianum, variant E282D== | |
| + | <StructureSection load='6gm2' size='340' side='right'caption='[[6gm2]], [[Resolution|resolution]] 2.76Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6gm2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_pasteurianum Clostridium pasteurianum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GM2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GM2 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.76Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=402:DICARBONYL[BIS(CYANIDE-KAPPAC)]-MU-(IMINODIMETHANETHIOLATATO-1KAPPAS 2KAPPAS)-MU-(OXOMETHYLIDENE)DIIRON(2+)'>402</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6gm2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gm2 OCA], [https://pdbe.org/6gm2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6gm2 RCSB], [https://www.ebi.ac.uk/pdbsum/6gm2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6gm2 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PHF1_CLOPA PHF1_CLOPA] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The unmatched catalytic turnover rates of [FeFe]-hydrogenases require an exceptionally efficient proton-transfer (PT) pathway to shuttle protons as substrates or products between bulk water and catalytic center. For clostridial [FeFe]-hydrogenase CpI such a pathway has been proposed and analyzed, but mainly on a theoretical basis. Here, eleven enzyme variants of two different [FeFe]-hydrogenases (CpI and HydA1) with substitutions in the presumptive PT-pathway are examined kinetically, spectroscopically, and crystallographically to provide solid experimental proof for its role in hydrogen-turnover. Targeting key residues of the PT-pathway by site directed mutagenesis significantly alters the pH-activity profile of these variants and in presence of H2 their cofactor is trapped in an intermediate state indicative of precluded proton-transfer. Furthermore, crystal structures coherently explain the individual levels of residual activity, demonstrating e.g. how trapped H2O molecules rescue the interrupted PT-pathway. These features provide conclusive evidence that the targeted positions are indeed vital for catalytic proton-transfer. | ||
| - | + | Crystallographic and spectroscopic assignment of the proton transfer pathway in [FeFe]-hydrogenases.,Duan J, Senger M, Esselborn J, Engelbrecht V, Wittkamp F, Apfel UP, Hofmann E, Stripp ST, Happe T, Winkler M Nat Commun. 2018 Nov 9;9(1):4726. doi: 10.1038/s41467-018-07140-x. PMID:30413719<ref>PMID:30413719</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6gm2" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: Happe | + | __TOC__ |
| - | [[Category: Hofmann | + | </StructureSection> |
| + | [[Category: Clostridium pasteurianum]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Duan J]] | ||
| + | [[Category: Esselborn J]] | ||
| + | [[Category: Happe T]] | ||
| + | [[Category: Hofmann E]] | ||
| + | [[Category: Winkler M]] | ||
Current revision
[FeFe]-hydrogenase CpI from Clostridium pasteurianum, variant E282D
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