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2isj

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[[Image:2isj.gif|left|200px]]
 
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{{Structure
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==BluB bound to oxidized FMN==
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|PDB= 2isj |SIZE=350|CAPTION= <scene name='initialview01'>2isj</scene>, resolution 2.3&Aring;
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<StructureSection load='2isj' size='340' side='right'caption='[[2isj]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>
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<table><tr><td colspan='2'>[[2isj]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Sinorhizobium_meliloti Sinorhizobium meliloti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ISJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ISJ FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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|GENE= bluB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=382 Sinorhizobium meliloti])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2isj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2isj OCA], [https://pdbe.org/2isj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2isj RCSB], [https://www.ebi.ac.uk/pdbsum/2isj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2isj ProSAT]</span></td></tr>
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|RELATEDENTRY=[[2isk|2ISK]], [[2isl|2ISL]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2isj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2isj OCA], [http://www.ebi.ac.uk/pdbsum/2isj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2isj RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/BLUB_RHIME BLUB_RHIME] Involved in the biosynthesis of cobalamin (vitamin B12). Catalyzes the oxidative fragmentation and contraction of the isoalloxazine heterocycle and the cleavage of the ribityl tail of FMN(2) to form 5,6-dimethylbenzimidazole (DMB) and D-erythrose 4-phosphate (E4P). NAD(P)H is only required initially to reduce FMN and oxygen drives the oxidative fragmentation.<ref>PMID:16537439</ref> <ref>PMID:17377583</ref>
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== Evolutionary Conservation ==
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'''BluB bound to oxidized FMN'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/is/2isj_consurf.spt"</scriptWhenChecked>
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An insight into a previously unknown step in B(12) biosynthesis was unexpectedly obtained through our analysis of a mutant of the symbiotic nitrogen fixing bacterium Sinorhizobium meliloti. This mutant was identified based on its unusually bright fluorescence on plates containing the succinoglycan binding dye calcofluor. The mutant contains a Tn5 insertion in a gene that has not been characterized previously in S. meliloti. The closest known homolog is the bluB gene of Rhodobacter capsulatus, which is implicated in the biosynthesis of B(12) (cobalamin). The S. meliloti bluB mutant is unable to grow in minimal media and fails to establish a symbiosis with alfalfa, and these defects can be rescued by the addition of vitamin B(12) (cyanocobalamin) or the lower ligand of cobalamin, 5,6-dimethylbenzimidazole (DMB). Biochemical analysis demonstrated that the bluB mutant does not produce cobalamin unless DMB is supplied. Sequence comparison suggests that BluB is a member of the NADH/flavin mononucleotide (FMN)-dependent nitroreductase family, and we propose that it is involved in the conversion of FMN to DMB.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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2ISJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sinorhizobium_meliloti Sinorhizobium meliloti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ISJ OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2isj ConSurf].
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<div style="clear:both"></div>
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==Reference==
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== References ==
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Sinorhizobium meliloti bluB is necessary for production of 5,6-dimethylbenzimidazole, the lower ligand of B12., Campbell GR, Taga ME, Mistry K, Lloret J, Anderson PJ, Roth JR, Walker GC, Proc Natl Acad Sci U S A. 2006 Mar 21;103(12):4634-9. Epub 2006 Mar 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16537439 16537439]
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<references/>
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[[Category: Single protein]]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Sinorhizobium meliloti]]
[[Category: Sinorhizobium meliloti]]
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[[Category: Howard-Jones, A R.]]
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[[Category: Howard-Jones AR]]
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[[Category: Larsen, N A.]]
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[[Category: Larsen NA]]
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[[Category: Taga, M E.]]
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[[Category: Taga ME]]
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[[Category: Walker, G C.]]
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[[Category: Walker GC]]
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[[Category: Walsh, C T.]]
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[[Category: Walsh CT]]
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[[Category: flavin]]
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[[Category: flavin destructase]]
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[[Category: monooxygenase]]
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[[Category: oxidoreductase]]
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[[Category: vitamin b12]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:48:01 2008''
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Current revision

BluB bound to oxidized FMN

PDB ID 2isj

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