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| | ==Solution structure of the SAP domain of human nuclear protein Hcc-1== | | ==Solution structure of the SAP domain of human nuclear protein Hcc-1== |
| - | <StructureSection load='2do1' size='340' side='right' caption='[[2do1]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2do1' size='340' side='right'caption='[[2do1]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2do1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DO1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2DO1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2do1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DO1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DO1 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HCC1, HSPC316 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2do1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2do1 OCA], [http://pdbe.org/2do1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2do1 RCSB], [http://www.ebi.ac.uk/pdbsum/2do1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2do1 ProSAT], [http://www.topsan.org/Proteins/RSGI/2do1 TOPSAN]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2do1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2do1 OCA], [https://pdbe.org/2do1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2do1 RCSB], [https://www.ebi.ac.uk/pdbsum/2do1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2do1 ProSAT], [https://www.topsan.org/Proteins/RSGI/2do1 TOPSAN]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SARNP_HUMAN SARNP_HUMAN]] Binds both single-stranded and double-stranded DNA with higher affinity for the single-stranded form. Specifically binds to scaffold/matrix attachment region DNA. Also binds single-stranded RNA. Enhances RNA unwinding activity of DDX39A. May participate in important transcriptional or translational control of cell growth, metabolism and carcinogenesis. Component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production.<ref>PMID:15338056</ref> <ref>PMID:17196963</ref> <ref>PMID:20844015</ref> | + | [https://www.uniprot.org/uniprot/SARNP_HUMAN SARNP_HUMAN] Binds both single-stranded and double-stranded DNA with higher affinity for the single-stranded form. Specifically binds to scaffold/matrix attachment region DNA. Also binds single-stranded RNA. Enhances RNA unwinding activity of DDX39A. May participate in important transcriptional or translational control of cell growth, metabolism and carcinogenesis. Component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production.<ref>PMID:15338056</ref> <ref>PMID:17196963</ref> <ref>PMID:20844015</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Inoue, M]] | + | [[Category: Large Structures]] |
| - | [[Category: Kigawa, T]] | + | [[Category: Inoue M]] |
| - | [[Category: Muto, Y]] | + | [[Category: Kigawa T]] |
| - | [[Category: Structural genomic]] | + | [[Category: Muto Y]] |
| - | [[Category: Shirouzu, M]] | + | [[Category: Shirouzu M]] |
| - | [[Category: Suzuki, S]] | + | [[Category: Suzuki S]] |
| - | [[Category: Terada, T]] | + | [[Category: Terada T]] |
| - | [[Category: Yokoyama, S]] | + | [[Category: Yokoyama S]] |
| - | [[Category: Gene regulation]]
| + | |
| - | [[Category: National project on protein structural and functional analyse]]
| + | |
| - | [[Category: Nppsfa]]
| + | |
| - | [[Category: Rsgi]]
| + | |
| - | [[Category: Sap domain]]
| + | |
| Structural highlights
Function
SARNP_HUMAN Binds both single-stranded and double-stranded DNA with higher affinity for the single-stranded form. Specifically binds to scaffold/matrix attachment region DNA. Also binds single-stranded RNA. Enhances RNA unwinding activity of DDX39A. May participate in important transcriptional or translational control of cell growth, metabolism and carcinogenesis. Component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production.[1] [2] [3]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
References
- ↑ Leaw CL, Ren EC, Choong ML. Hcc-1 is a novel component of the nuclear matrix with growth inhibitory function. Cell Mol Life Sci. 2004 Sep;61(17):2264-73. PMID:15338056 doi:http://dx.doi.org/10.1007/s00018-004-4205-x
- ↑ Sugiura T, Sakurai K, Nagano Y. Intracellular characterization of DDX39, a novel growth-associated RNA helicase. Exp Cell Res. 2007 Feb 15;313(4):782-90. Epub 2006 Dec 5. PMID:17196963 doi:http://dx.doi.org/S0014-4827(06)00486-1
- ↑ Dufu K, Livingstone MJ, Seebacher J, Gygi SP, Wilson SA, Reed R. ATP is required for interactions between UAP56 and two conserved mRNA export proteins, Aly and CIP29, to assemble the TREX complex. Genes Dev. 2010 Sep 15;24(18):2043-53. doi: 10.1101/gad.1898610. PMID:20844015 doi:http://dx.doi.org/10.1101/gad.1898610
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