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| | ==Solution structure of the first SURP domain of human splicing factor SF3a120== | | ==Solution structure of the first SURP domain of human splicing factor SF3a120== |
| - | <StructureSection load='2dt6' size='340' side='right' caption='[[2dt6]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2dt6' size='340' side='right'caption='[[2dt6]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2dt6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DT6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2DT6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2dt6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DT6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DT6 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2dt7|2dt7]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SF3a120, SF3a1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2dt6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dt6 OCA], [https://pdbe.org/2dt6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2dt6 RCSB], [https://www.ebi.ac.uk/pdbsum/2dt6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2dt6 ProSAT], [https://www.topsan.org/Proteins/RSGI/2dt6 TOPSAN]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dt6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dt6 OCA], [http://pdbe.org/2dt6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2dt6 RCSB], [http://www.ebi.ac.uk/pdbsum/2dt6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2dt6 ProSAT], [http://www.topsan.org/Proteins/RSGI/2dt6 TOPSAN]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SF3A1_HUMAN SF3A1_HUMAN]] Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. | + | [https://www.uniprot.org/uniprot/SF3A1_HUMAN SF3A1_HUMAN] Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Guentert, P]] | + | [[Category: Large Structures]] |
| - | [[Category: He, F]] | + | [[Category: Guentert P]] |
| - | [[Category: Inoue, M]] | + | [[Category: He F]] |
| - | [[Category: Kuwasako, K]] | + | [[Category: Inoue M]] |
| - | [[Category: Muto, Y]] | + | [[Category: Kuwasako K]] |
| - | [[Category: Structural genomic]]
| + | [[Category: Muto Y]] |
| - | [[Category: Sugano, S]] | + | [[Category: Sugano S]] |
| - | [[Category: Tanaka, A]] | + | [[Category: Tanaka A]] |
| - | [[Category: Yokoyama, S]] | + | [[Category: Yokoyama S]] |
| - | [[Category: National project on protein structural and functional analyse]]
| + | |
| - | [[Category: Nppsfa]]
| + | |
| - | [[Category: Rna binding protein]]
| + | |
| - | [[Category: Rsgi]]
| + | |
| - | [[Category: Sf3a120]]
| + | |
| - | [[Category: Surp domain]]
| + | |
| Structural highlights
Function
SF3A1_HUMAN Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The SF3a complex, consisting of SF3a60, SF3a66, and SF3a120, in 17S U2 snRNP is crucial to spliceosomal assembly. SF3a120 contains two tandem SURP domains (SURP1 and SURP2), and SURP2 is responsible for binding to SF3a60. We found that the SURP2 fragment forms a stable complex with an SF3a60 fragment (residues 71-107) and solved its structure by NMR spectroscopy. SURP2 exhibits a fold of the alpha1-alpha2-3(10)-alpha3 topology, and the SF3a60 fragment forms an amphipathic alpha helix intimately contacting alpha1 of SURP2. We also solved the SURP1 structure, which has the same fold as SURP2. The protein-binding interface of SURP2 is quite similar to the corresponding surface of SURP1, except for two amino acid residues. One of them, Leu169, is characteristic of SF3a120 SURP2 among SURP domains. Mutagenesis showed that this single Leu residue is the critical determinant for complex formation, which reveals the protein recognition mechanism in the subunit assembly.
Solution structures of the SURP domains and the subunit-assembly mechanism within the splicing factor SF3a complex in 17S U2 snRNP.,Kuwasako K, He F, Inoue M, Tanaka A, Sugano S, Guntert P, Muto Y, Yokoyama S Structure. 2006 Nov;14(11):1677-89. PMID:17098193[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kuwasako K, He F, Inoue M, Tanaka A, Sugano S, Guntert P, Muto Y, Yokoyama S. Solution structures of the SURP domains and the subunit-assembly mechanism within the splicing factor SF3a complex in 17S U2 snRNP. Structure. 2006 Nov;14(11):1677-89. PMID:17098193 doi:10.1016/j.str.2006.09.009
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