5zz1
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5zz1 is ON HOLD until Paper Publication Authors: Yuzugullu Karakus, Y., Trinh, C.H., Pearson, A.R., Ogel, Z.B., McPherson, M.J. Description: Probin...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Probing the active center of catalase-phenol oxidase from Scytalidium thermophilum== | |
| + | <StructureSection load='5zz1' size='340' side='right'caption='[[5zz1]], [[Resolution|resolution]] 1.91Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5zz1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycothermus_thermophilus Mycothermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZZ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZZ1 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.91Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3TR:3-AMINO-1,2,4-TRIAZOLE'>3TR</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HDD:CIS-HEME+D+HYDROXYCHLORIN+GAMMA-SPIROLACTONE'>HDD</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zz1 OCA], [https://pdbe.org/5zz1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zz1 RCSB], [https://www.ebi.ac.uk/pdbsum/5zz1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zz1 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/M4GGR7_9PEZI M4GGR7_9PEZI] Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide.[RuleBase:RU004142] Serves to protect cells from the toxic effects of hydrogen peroxide.[PIRNR:PIRNR038927] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The catalase from Scytalidium thermophilum is a homotetramer containing a heme d in each active site. Although the enzyme has a classical monofunctional catalase fold, it also possesses oxidase activity towards a number of small organics, including catechol and phenol. In order to further investigate this, the crystal structure of the complex of the catalase with the classical catalase inhibitor 3-amino-1,2,4-triazole (3TR) was determined at 1.95 A resolution. Surprisingly, no binding to the heme site was observed; instead, 3TR occupies a binding site corresponding to the NADPH-binding pocket in mammalian catalases at the entrance to a lateral channel leading to the heme. Kinetic analysis of site-directed mutants supports the assignment of this pocket as the binding site for oxidase substrates. | ||
| - | + | Identification of the site of oxidase substrate binding in Scytalidium thermophilum catalase.,Yuzugullu Karakus Y, Goc G, Balci S, Yorke BA, Trinh CH, McPherson MJ, Pearson AR Acta Crystallogr D Struct Biol. 2018 Oct 1;74(Pt 10):979-985. doi:, 10.1107/S2059798318010628. Epub 2018 Oct 2. PMID:30289408<ref>PMID:30289408</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5zz1" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| - | [[Category: | + | ==See Also== |
| - | [[Category: | + | *[[Catalase 3D structures|Catalase 3D structures]] |
| - | [[Category: Trinh | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Mycothermus thermophilus]] | ||
| + | [[Category: McPherson MJ]] | ||
| + | [[Category: Ogel ZB]] | ||
| + | [[Category: Pearson AR]] | ||
| + | [[Category: Trinh CH]] | ||
| + | [[Category: Yuzugullu Karakus Y]] | ||
Current revision
Probing the active center of catalase-phenol oxidase from Scytalidium thermophilum
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