6gnz
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6gnz is ON HOLD Authors: Ekblad, B., Kristiansen, P.E. Description: Plantaricin S-b in 100 mM DPC micelles. This is the alfa part of the bacterioci...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Plantaricin S-a in 100 mM DPC micelles. This is the alpha part of the bacteriocin plantaricin S.== | |
| + | <StructureSection load='6gnz' size='340' side='right'caption='[[6gnz]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6gnz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactiplantibacillus_plantarum Lactiplantibacillus plantarum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GNZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GNZ FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6gnz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gnz OCA], [https://pdbe.org/6gnz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6gnz RCSB], [https://www.ebi.ac.uk/pdbsum/6gnz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6gnz ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/O32831_LACPN O32831_LACPN] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The structure of the individual peptides of the two-peptide bacteriocin plantaricin S, an antimicrobial peptide produced by a Lactobacillus plantarum strain, has been determined in DPC micelles. The two peptides of plantaricin S, Pls-alpha and Pls-beta, form an alpha-helix from and including residue 8 to 24 with a less structured region around residue 16-19 and an amphiphilic alpha-helix from and including residue 7 to 23, respectively. Activity assays on single amino acid-substituted GxxxG and GxxxG-like motifs show that substituting the Ser and Gly residues in the G9xxxG13 motif in Pls-alpha and the S17xxxG21 motif in Pls-beta reduced or drastically reduced the antimicrobial activity. The two-peptide bacteriocin muricidin contains GxxxG-like motifs at similar positions and displays 40-50% amino acid identity with plantaricin S. Activity assays of combinations of the peptides that constitute the bacteriocins plantaricin S and muricidin show that some combinations are highly active. Furthermore, sequence alignments show that the motifs important for plantaricin S activity align with identical motifs in muricidin. Based on sequence comparison and activity assays, a membrane-inserted model of plantaricin S in which the two peptides are oriented antiparallel relative to each other and where the GxxxG and GxxxG-like motifs important for activity come close in space, is proposed. | ||
| - | + | NMR structures and mutational analysis of the two peptides constituting the bacteriocin plantaricin S.,Ekblad B, Kristiansen PE Sci Rep. 2019 Feb 20;9(1):2333. doi: 10.1038/s41598-019-38518-6. PMID:30787405<ref>PMID:30787405</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Ekblad | + | <div class="pdbe-citations 6gnz" style="background-color:#fffaf0;"></div> |
| - | [[Category: Kristiansen | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Lactiplantibacillus plantarum]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Ekblad B]] | ||
| + | [[Category: Kristiansen PE]] | ||
Current revision
Plantaricin S-a in 100 mM DPC micelles. This is the alpha part of the bacteriocin plantaricin S.
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