This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2j04

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (11:23, 31 March 2021) (edit) (undo)
 
(11 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2j04.jpg|left|200px]]
 
-
{{Structure
+
==The tau60-tau91 subcomplex of yeast transcription factor IIIC==
-
|PDB= 2j04 |SIZE=350|CAPTION= <scene name='initialview01'>2j04</scene>, resolution 3.20&Aring;
+
<StructureSection load='2j04' size='340' side='right'caption='[[2j04]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND=
+
<table><tr><td colspan='2'>[[2j04]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J04 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J04 FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j04 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j04 OCA], [https://pdbe.org/2j04 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j04 RCSB], [https://www.ebi.ac.uk/pdbsum/2j04 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j04 ProSAT]</span></td></tr>
-
|GENE=
+
</table>
-
|DOMAIN=
+
== Function ==
-
|RELATEDENTRY=
+
[[https://www.uniprot.org/uniprot/TFC8_YEAST TFC8_YEAST]] TFIIIC mediates tRNA and 5S RNA gene activation by binding to intragenic promoter elements. Upstream of the transcription start site, TFIIIC assembles the initiation complex TFIIIB-TFIIIC-tDNA, which is sufficient for RNA polymerase III recruitment and function. Part of the tauB domain of TFIIIC that binds boxB DNA promoter sites of tRNA and similar genes. Plays a role in TFIIB assembly through its interaction with SPT15/TBP. Essential for cell viability.<ref>PMID:10567530</ref>
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j04 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j04 OCA], [http://www.ebi.ac.uk/pdbsum/2j04 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2j04 RCSB]</span>
+
== Evolutionary Conservation ==
-
}}
+
[[Image:Consurf_key_small.gif|200px|right]]
-
 
+
Check<jmol>
-
'''THE TAU60-TAU91 SUBCOMPLEX OF YEAST TRANSCRIPTION FACTOR IIIC'''
+
<jmolCheckbox>
-
 
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j0/2j04_consurf.spt"</scriptWhenChecked>
-
 
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
==Overview==
+
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j04 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
Yeast RNA polymerase III is recruited upon binding of subcomplexes tauA and tauB of transcription factor IIIC (TFIIIC) to the A and B blocks of tRNA gene promoters. The tauB subcomplex consists of subunits tau60, tau91, and tau138. We determined the 3.2 A crystal structure of tau60 bound to a large C-terminal fragment of tau91 (Deltatau91). Deltatau91 protein contains a seven-bladed propeller preceded by an N-terminal extension, whereas tau60 contains a structurally homologous propeller followed by a C-terminal domain with a novel alpha/beta fold. The two propeller domains do not have any detectable DNA binding activity and mediate heterodimer formation that may serve as scaffold for tau138 assembly. We show that the C-terminal tau60 domain interacts with the TATA binding protein (TBP). Recombinant tauB recruits TBP and stimulates TFIIIB-directed transcription on a TATA box containing tRNA gene, implying a combined contribution of tauA and tauB to preinitiation complex formation.
Yeast RNA polymerase III is recruited upon binding of subcomplexes tauA and tauB of transcription factor IIIC (TFIIIC) to the A and B blocks of tRNA gene promoters. The tauB subcomplex consists of subunits tau60, tau91, and tau138. We determined the 3.2 A crystal structure of tau60 bound to a large C-terminal fragment of tau91 (Deltatau91). Deltatau91 protein contains a seven-bladed propeller preceded by an N-terminal extension, whereas tau60 contains a structurally homologous propeller followed by a C-terminal domain with a novel alpha/beta fold. The two propeller domains do not have any detectable DNA binding activity and mediate heterodimer formation that may serve as scaffold for tau138 assembly. We show that the C-terminal tau60 domain interacts with the TATA binding protein (TBP). Recombinant tauB recruits TBP and stimulates TFIIIB-directed transcription on a TATA box containing tRNA gene, implying a combined contribution of tauA and tauB to preinitiation complex formation.
-
==About this Structure==
+
Structure of the tau60/Delta tau91 subcomplex of yeast transcription factor IIIC: insights into preinitiation complex assembly.,Mylona A, Fernandez-Tornero C, Legrand P, Haupt M, Sentenac A, Acker J, Muller CW Mol Cell. 2006 Oct 20;24(2):221-32. PMID:17052456<ref>PMID:17052456</ref>
-
2J04 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J04 OCA].
+
-
 
+
-
==Reference==
+
-
Structure of the tau60/Delta tau91 subcomplex of yeast transcription factor IIIC: insights into preinitiation complex assembly., Mylona A, Fernandez-Tornero C, Legrand P, Haupt M, Sentenac A, Acker J, Muller CW, Mol Cell. 2006 Oct 20;24(2):221-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17052456 17052456]
+
-
[[Category: Protein complex]]
+
-
[[Category: Saccharomyces cerevisiae]]
+
-
[[Category: Fernandez-Tornero, C.]]
+
-
[[Category: Legrand, P.]]
+
-
[[Category: Muller, C W.]]
+
-
[[Category: Mylona, A.]]
+
-
[[Category: beta propeller]]
+
-
[[Category: hypothetical protein]]
+
-
[[Category: preinitiation complex]]
+
-
[[Category: transcription]]
+
-
[[Category: transcription factor iiic]]
+
-
[[Category: type 2 promoter]]
+
-
[[Category: yeast rna polymerase iii]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:51:11 2008''
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 2j04" style="background-color:#fffaf0;"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Atcc 18824]]
 +
[[Category: Large Structures]]
 +
[[Category: Fernandez-Tornero, C]]
 +
[[Category: Legrand, P]]
 +
[[Category: Muller, C W]]
 +
[[Category: Mylona, A]]
 +
[[Category: Beta propeller]]
 +
[[Category: Hypothetical protein]]
 +
[[Category: Preinitiation complex]]
 +
[[Category: Transcription]]
 +
[[Category: Transcription factor iiic]]
 +
[[Category: Type 2 promoter]]
 +
[[Category: Yeast rna polymerase iii]]

Current revision

The tau60-tau91 subcomplex of yeast transcription factor IIIC

PDB ID 2j04

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2j04"
Personal tools