6eyx

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==Anti-CRISPR AcrIIa6 tetragonal form==
==Anti-CRISPR AcrIIa6 tetragonal form==
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<StructureSection load='6eyx' size='340' side='right' caption='[[6eyx]], [[Resolution|resolution]] 1.96&Aring;' scene=''>
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<StructureSection load='6eyx' size='340' side='right'caption='[[6eyx]], [[Resolution|resolution]] 1.96&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6eyx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EYX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EYX FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6eyx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_virus_DT1 Streptococcus virus DT1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EYX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6EYX FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6eyx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eyx OCA], [http://pdbe.org/6eyx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6eyx RCSB], [http://www.ebi.ac.uk/pdbsum/6eyx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6eyx ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.96&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6eyx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eyx OCA], [https://pdbe.org/6eyx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6eyx RCSB], [https://www.ebi.ac.uk/pdbsum/6eyx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6eyx ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9XJC1_9CAUD Q9XJC1_9CAUD]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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CRISPR-Cas systems are bacterial anti-viral systems, and bacterial viruses (bacteriophages, phages) can carry anti-CRISPR (Acr) proteins to evade that immunity. Acrs can also fine-tune the activity of CRISPR-based genome-editing tools. While Acrs are prevalent in phages capable of lying dormant in a CRISPR-carrying host, their orthologs have been observed only infrequently in virulent phages. Here we identify AcrIIA6, an Acr encoded in 33% of virulent Streptococcus thermophilus phage genomes. The X-ray structure of AcrIIA6 displays some features unique to this Acr family. We compare the activity of AcrIIA6 to those of other Acrs, including AcrIIA5 (also from S. thermophilus phages), and characterize their effectiveness against a range of CRISPR-Cas systems. Finally, we demonstrate that both Acr families from S. thermophilus phages inhibit Cas9-mediated genome editing of human cells.
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Widespread anti-CRISPR proteins in virulent bacteriophages inhibit a range of Cas9 proteins.,Hynes AP, Rousseau GM, Agudelo D, Goulet A, Amigues B, Loehr J, Romero DA, Fremaux C, Horvath P, Doyon Y, Cambillau C, Moineau S Nat Commun. 2018 Jul 25;9(1):2919. doi: 10.1038/s41467-018-05092-w. PMID:30046034<ref>PMID:30046034</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6eyx" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Amigues, B]]
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[[Category: Large Structures]]
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[[Category: Cambillau, C]]
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[[Category: Streptococcus virus DT1]]
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[[Category: Moineau, S]]
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[[Category: Amigues B]]
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[[Category: Anti-crispr]]
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[[Category: Cambillau C]]
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[[Category: Dna binding]]
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[[Category: Moineau S]]
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[[Category: Hth fold]]
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[[Category: Viral protein]]
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Current revision

Anti-CRISPR AcrIIa6 tetragonal form

PDB ID 6eyx

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