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| | ==CRYSTAL STRUCTURE ANALYSIS OF ALDOLASE FROM T. BRUCEI== | | ==CRYSTAL STRUCTURE ANALYSIS OF ALDOLASE FROM T. BRUCEI== |
| - | <StructureSection load='1f2j' size='340' side='right' caption='[[1f2j]], [[Resolution|resolution]] 1.90Å' scene=''> | + | <StructureSection load='1f2j' size='340' side='right'caption='[[1f2j]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1f2j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_(trypanozoon)_brucei Trypanosoma (trypanozoon) brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F2J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1F2J FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1f2j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei Trypanosoma brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F2J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F2J FirstGlance]. <br> |
| - | </td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructose-bisphosphate_aldolase Fructose-bisphosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.13 4.1.2.13] </span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f2j OCA], [http://pdbe.org/1f2j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1f2j RCSB], [http://www.ebi.ac.uk/pdbsum/1f2j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1f2j ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f2j OCA], [https://pdbe.org/1f2j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f2j RCSB], [https://www.ebi.ac.uk/pdbsum/1f2j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f2j ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/ALF_TRYBB ALF_TRYBB] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | ==See Also== | | ==See Also== |
| - | *[[Aldolase|Aldolase]] | + | *[[Aldolase 3D structures|Aldolase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Fructose-bisphosphate aldolase]] | + | [[Category: Large Structures]] |
| - | [[Category: Chudzik, D M]] | + | [[Category: Trypanosoma brucei]] |
| - | [[Category: Hol, W G.J]] | + | [[Category: Chudzik DM]] |
| - | [[Category: Michels, P A]]
| + | [[Category: De Walque S]] |
| - | [[Category: Walque, S De]] | + | [[Category: Hol WGJ]] |
| - | [[Category: Bisphosphate]] | + | [[Category: Michels PA]] |
| - | [[Category: Aldolase]] | + | |
| - | [[Category: Beta barrel]]
| + | |
| - | [[Category: Fructose 1]]
| + | |
| - | [[Category: Lyase]]
| + | |
| - | [[Category: Pts signal]]
| + | |
| - | [[Category: Trypanosoma]]
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| Structural highlights
Function
ALF_TRYBB
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Trypanosomatids, unicellular organisms responsible for several global diseases, contain unique organelles called glycosomes in which the first seven glycolytic enzymes are sequestered. We report the crystal structures of glycosomal fructose-1,6-bisphosphate aldolase from two major tropical pathogens, Trypanosoma brucei and Leishmania mexicana, the causative agents of African sleeping sickness and one form of leishmaniasis, respectively. Unlike mammalian aldolases, the T. brucei and L. mexicana aldolases contain nonameric N-terminal type 2 peroxisomal targeting signals (PTS2s) to direct their import into the glycosome. In both tetrameric trypanosomatid aldolases, the PTS2s from two different subunits form two closely intertwined structures. These "PTS2 dimers", which have very similar conformations in the two aldolase structures, are the first reported conformations of a glycosomal or peroxisomal PTS2, and provide opportunities for the design of trypanocidal compounds.
Structures of type 2 peroxisomal targeting signals in two trypanosomatid aldolases.,Chudzik DM, Michels PA, de Walque S, Hol WG J Mol Biol. 2000 Jul 21;300(4):697-707. PMID:10891264[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Chudzik DM, Michels PA, de Walque S, Hol WG. Structures of type 2 peroxisomal targeting signals in two trypanosomatid aldolases. J Mol Biol. 2000 Jul 21;300(4):697-707. PMID:10891264 doi:10.1006/jmbi.2000.3910
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