6bbr
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==Room temperature neutron/X-ray structure of AAC-VIa== | ==Room temperature neutron/X-ray structure of AAC-VIa== | ||
| - | <StructureSection load='6bbr' size='340' side='right' caption='[[6bbr]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='6bbr' size='340' side='right'caption='[[6bbr]], [[Resolution|resolution]] 2.00Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6bbr]] is a 1 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[6bbr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacter_cloacae Enterobacter cloacae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BBR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BBR FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Hybrid , Neutron Diffraction , X-ray diffraction, [[Resolution|Resolution]] 2.002Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DOD:DEUTERATED+WATER'>DOD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | < | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bbr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bbr OCA], [https://pdbe.org/6bbr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bbr RCSB], [https://www.ebi.ac.uk/pdbsum/6bbr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bbr ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | |
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q47030_ENTCL Q47030_ENTCL] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | One group of enzymes that confer resistance to aminoglycoside antibiotics through covalent modification belongs to the GCN5-related N-acetyltransferase (GNAT) superfamily. We show how a unique GNAT subfamily member uses a previously unidentified noncanonical catalytic triad, consisting of a glutamic acid, a histidine, and the antibiotic substrate itself, which acts as a nucleophile and attacks the acetyl donor molecule. Neutron diffraction studies allow for unambiguous identification of a low-barrier hydrogen bond, predicted in canonical catalytic triads to increase basicity of the histidine. This work highlights the role of this unique catalytic triad in mediating antibiotic resistance while providing new insights into the design of the next generation of aminoglycosides. | ||
| + | |||
| + | A low-barrier hydrogen bond mediates antibiotic resistance in a noncanonical catalytic triad.,Kumar P, Serpersu EH, Cuneo MJ Sci Adv. 2018 Apr 4;4(4):eaas8667. doi: 10.1126/sciadv.aas8667. eCollection 2018 , Apr. PMID:29632894<ref>PMID:29632894</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6bbr" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Enterobacter cloacae]] |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Cuneo MJ]] |
| - | [[Category: | + | [[Category: Kumar P]] |
| - | [[Category: | + | [[Category: Serpersu EH]] |
Current revision
Room temperature neutron/X-ray structure of AAC-VIa
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