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| | ==Crystal structure of intracellular family 1 beta-glucosidase BGL1A from the basidiomycete Phanerochaete chrysosporium in substrate-free form== | | ==Crystal structure of intracellular family 1 beta-glucosidase BGL1A from the basidiomycete Phanerochaete chrysosporium in substrate-free form== |
| - | <StructureSection load='2e3z' size='340' side='right' caption='[[2e3z]], [[Resolution|resolution]] 1.50Å' scene=''> | + | <StructureSection load='2e3z' size='340' side='right'caption='[[2e3z]], [[Resolution|resolution]] 1.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2e3z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chrysosporium_pruinosum_(gilman_&_abbott)_carmich. Chrysosporium pruinosum (gilman & abbott) carmich.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E3Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2E3Z FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2e3z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Phanerodontia_chrysosporium Phanerodontia chrysosporium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E3Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2E3Z FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2e40|2e40]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bgl1A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5306 Chrysosporium pruinosum (Gilman & Abbott) Carmich.])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2e3z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e3z OCA], [https://pdbe.org/2e3z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2e3z RCSB], [https://www.ebi.ac.uk/pdbsum/2e3z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2e3z ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2e3z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e3z OCA], [http://pdbe.org/2e3z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2e3z RCSB], [http://www.ebi.ac.uk/pdbsum/2e3z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2e3z ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/BGL1A_PHACH BGL1A_PHACH]] Plays an important role in cellulose degradation. Shows hydrolytic activity against several glycosidic compounds.<ref>PMID:16896601</ref> | + | [https://www.uniprot.org/uniprot/BGL1A_PHACH BGL1A_PHACH] Plays an important role in cellulose degradation. Shows hydrolytic activity against several glycosidic compounds.<ref>PMID:16896601</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | ==See Also== | | ==See Also== |
| - | *[[Beta-glucosidase|Beta-glucosidase]] | + | *[[Beta-glucosidase 3D structures|Beta-glucosidase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Beta-glucosidase]] | + | [[Category: Large Structures]] |
| - | [[Category: Fushinobu, S]] | + | [[Category: Phanerodontia chrysosporium]] |
| - | [[Category: Igarashi, K]] | + | [[Category: Fushinobu S]] |
| - | [[Category: Nijikken, Y]] | + | [[Category: Igarashi K]] |
| - | [[Category: Samejima, M]] | + | [[Category: Nijikken Y]] |
| - | [[Category: Tsukada, T]] | + | [[Category: Samejima M]] |
| - | [[Category: Clan gh-a]]
| + | [[Category: Tsukada T]] |
| - | [[Category: Glycoside hydrolase family 1]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: National project on protein structural and functional analyse]]
| + | |
| - | [[Category: Nppsfa]]
| + | |
| - | [[Category: Structural genomic]]
| + | |
| - | [[Category: Tim barrel]]
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| Structural highlights
Function
BGL1A_PHACH Plays an important role in cellulose degradation. Shows hydrolytic activity against several glycosidic compounds.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The white-rot fungus Phanerochaete chrysosporium has two intracellular beta-glucosidases (BGL1A and BGL1B) belonging to glycoside hydrolase (GH) family 1. BGL1B effectively hydrolyzes cellobiose and cellobionolactone, but BGL1A does not. We have determined the crystal structure of BGL1A in substrate-free and gluconolactone complexed forms. The overall structure and the characteristic of subsite -1 (glycone site) were similar to those of other known GH1 enzymes. The loop regions covering on the (beta/alpha)(8) barrel was significantly deviated, and they form a unique subsite +1 (aglycone site) of BGL1A.
Crystal structure of intracellular family 1 beta-glucosidase BGL1A from the basidiomycete Phanerochaete chrysosporium.,Nijikken Y, Tsukada T, Igarashi K, Samejima M, Wakagi T, Shoun H, Fushinobu S FEBS Lett. 2007 Apr 3;581(7):1514-20. Epub 2007 Mar 13. PMID:17376440[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Tsukada T, Igarashi K, Yoshida M, Samejima M. Molecular cloning and characterization of two intracellular beta-glucosidases belonging to glycoside hydrolase family 1 from the basidiomycete Phanerochaete chrysosporium. Appl Microbiol Biotechnol. 2006 Dec;73(4):807-14. Epub 2006 Aug 9. PMID:16896601 doi:10.1007/s00253-006-0526-z
- ↑ Nijikken Y, Tsukada T, Igarashi K, Samejima M, Wakagi T, Shoun H, Fushinobu S. Crystal structure of intracellular family 1 beta-glucosidase BGL1A from the basidiomycete Phanerochaete chrysosporium. FEBS Lett. 2007 Apr 3;581(7):1514-20. Epub 2007 Mar 13. PMID:17376440 doi:10.1016/j.febslet.2007.03.009
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