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2j3w

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[[Image:2j3w.jpg|left|200px]]
 
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{{Structure
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==The crystal structure of the bet3-trs31-sedlin complex.==
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|PDB= 2j3w |SIZE=350|CAPTION= <scene name='initialview01'>2j3w</scene>, resolution 2.1&Aring;
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<StructureSection load='2j3w' size='340' side='right'caption='[[2j3w]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=P1L:S-PALMITOYL-L-CYSTEINE'>P1L</scene>
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<table><tr><td colspan='2'>[[2j3w]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Brachidanio_rerio Brachidanio rerio] and [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J3W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J3W FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
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|GENE=
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2j3r|2j3r]], [[2j3t|2j3t]]</div></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j3w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j3w OCA], [https://pdbe.org/2j3w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j3w RCSB], [https://www.ebi.ac.uk/pdbsum/2j3w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j3w ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j3w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j3w OCA], [http://www.ebi.ac.uk/pdbsum/2j3w PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2j3w RCSB]</span>
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== Evolutionary Conservation ==
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}}
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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'''THE CRYSTAL STRUCTURE OF THE BET3-TRS31-SEDLIN COMPLEX.'''
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j3/2j3w_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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==Overview==
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j3w ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
Transport protein particle (TRAPP) I is a multisubunit vesicle tethering factor composed of seven subunits involved in ER-to-Golgi trafficking. The functional mechanism of the complex and how the subunits interact to form a functional unit are unknown. Here, we have used a multidisciplinary approach that includes X-ray crystallography, electron microscopy, biochemistry, and yeast genetics to elucidate the architecture of TRAPP I. The complex is organized through lateral juxtaposition of the subunits into a flat and elongated particle. We have also localized the site of guanine nucleotide exchange activity to a highly conserved surface encompassing several subunits. We propose that TRAPP I attaches to Golgi membranes with its large flat surface containing many highly conserved residues and forms a platform for protein-protein interactions. This study provides the most comprehensive view of a multisubunit vesicle tethering complex to date, based on which a model for the function of this complex, involving Rab1-GTP and long, coiled-coil tethers, is presented.
Transport protein particle (TRAPP) I is a multisubunit vesicle tethering factor composed of seven subunits involved in ER-to-Golgi trafficking. The functional mechanism of the complex and how the subunits interact to form a functional unit are unknown. Here, we have used a multidisciplinary approach that includes X-ray crystallography, electron microscopy, biochemistry, and yeast genetics to elucidate the architecture of TRAPP I. The complex is organized through lateral juxtaposition of the subunits into a flat and elongated particle. We have also localized the site of guanine nucleotide exchange activity to a highly conserved surface encompassing several subunits. We propose that TRAPP I attaches to Golgi membranes with its large flat surface containing many highly conserved residues and forms a platform for protein-protein interactions. This study provides the most comprehensive view of a multisubunit vesicle tethering complex to date, based on which a model for the function of this complex, involving Rab1-GTP and long, coiled-coil tethers, is presented.
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==About this Structure==
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The architecture of the multisubunit TRAPP I complex suggests a model for vesicle tethering.,Kim YG, Raunser S, Munger C, Wagner J, Song YL, Cygler M, Walz T, Oh BH, Sacher M Cell. 2006 Nov 17;127(4):817-30. PMID:17110339<ref>PMID:17110339</ref>
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2J3W is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Danio_rerio Danio rerio] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J3W OCA].
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==Reference==
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The architecture of the multisubunit TRAPP I complex suggests a model for vesicle tethering., Kim YG, Raunser S, Munger C, Wagner J, Song YL, Cygler M, Walz T, Oh BH, Sacher M, Cell. 2006 Nov 17;127(4):817-30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17110339 17110339]
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[[Category: Danio rerio]]
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[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Kim, Y G.]]
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[[Category: Oh, B H.]]
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[[Category: endoplasmic reticulum]]
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[[Category: er-golgi transport]]
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[[Category: golgi apparatus]]
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[[Category: lipoprotein]]
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[[Category: multisubunit tethering factor]]
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[[Category: palmitate]]
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[[Category: transcription]]
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[[Category: transport]]
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[[Category: trapp]]
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[[Category: vesicle transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:52:35 2008''
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2j3w" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Brachidanio rerio]]
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[[Category: Large Structures]]
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[[Category: Lk3 transgenic mice]]
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[[Category: Kim, Y G]]
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[[Category: Oh, B H]]
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[[Category: Endoplasmic reticulum]]
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[[Category: Er-golgi transport]]
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[[Category: Golgi apparatus]]
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[[Category: Lipoprotein]]
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[[Category: Multisubunit tethering factor]]
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[[Category: Palmitate]]
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[[Category: Transcription]]
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[[Category: Transport]]
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[[Category: Trapp]]
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[[Category: Vesicle transport]]

Current revision

The crystal structure of the bet3-trs31-sedlin complex.

PDB ID 2j3w

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