|
|
| (One intermediate revision not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Crystal structure of ribosome-binding factor A from Thermus thermophilus HB8== | | ==Crystal structure of ribosome-binding factor A from Thermus thermophilus HB8== |
| - | <StructureSection load='2dyj' size='340' side='right' caption='[[2dyj]], [[Resolution|resolution]] 1.84Å' scene=''> | + | <StructureSection load='2dyj' size='340' side='right'caption='[[2dyj]], [[Resolution|resolution]] 1.84Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2dyj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DYJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2DYJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2dyj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DYJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DYJ FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TTHA0907 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.84Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dyj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dyj OCA], [http://pdbe.org/2dyj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2dyj RCSB], [http://www.ebi.ac.uk/pdbsum/2dyj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2dyj ProSAT], [http://www.topsan.org/Proteins/RSGI/2dyj TOPSAN]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2dyj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dyj OCA], [https://pdbe.org/2dyj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2dyj RCSB], [https://www.ebi.ac.uk/pdbsum/2dyj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2dyj ProSAT], [https://www.topsan.org/Proteins/RSGI/2dyj TOPSAN]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/RBFA_THET8 RBFA_THET8] One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes) (PubMed:17996707). Required for efficient processing of 16S rRNA. Probably interacts with the 5'-terminal helix region of 16S rRNA, bringing together different domains of the 30S ribosomal subunit which aids assembly (PubMed:17996707).<ref>PMID:17996707</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| Line 30: |
Line 32: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Thet8]] | + | [[Category: Large Structures]] |
| - | [[Category: Kawazoe, M]] | + | [[Category: Thermus thermophilus HB8]] |
| - | [[Category: Nakayama-Ushikoshi, R]] | + | [[Category: Kawazoe M]] |
| - | [[Category: Structural genomic]] | + | [[Category: Nakayama-Ushikoshi R]] |
| - | [[Category: Shirouzu, M]] | + | [[Category: Shirouzu M]] |
| - | [[Category: Takemoto, C]] | + | [[Category: Takemoto C]] |
| - | [[Category: Terada, T]] | + | [[Category: Terada T]] |
| - | [[Category: Yokoyama, S]] | + | [[Category: Yokoyama S]] |
| - | [[Category: 16s rrna processing]]
| + | |
| - | [[Category: 17s rna]]
| + | |
| - | [[Category: Kh domain]]
| + | |
| - | [[Category: National project on protein structural and functional analyse]]
| + | |
| - | [[Category: Nppsfa]]
| + | |
| - | [[Category: Ribosomal protein]]
| + | |
| - | [[Category: Rsgi]]
| + | |
| Structural highlights
Function
RBFA_THET8 One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes) (PubMed:17996707). Required for efficient processing of 16S rRNA. Probably interacts with the 5'-terminal helix region of 16S rRNA, bringing together different domains of the 30S ribosomal subunit which aids assembly (PubMed:17996707).[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Ribosome binding factor A (RbfA) is a bacterial cold shock response protein, required for an efficient processing of the 5' end of the 16S ribosomal RNA (rRNA) during assembly of the small (30S) ribosomal subunit. Here we present a crystal structure of Thermus thermophilus (Tth) RbfA and a three-dimensional cryo-electron microscopic (EM) map of the Tth 30S*RbfA complex. RbfA binds to the 30S subunit in a position overlapping the binding sites of the A and P site tRNAs, and RbfA's functionally important C terminus extends toward the 5' end of the 16S rRNA. In the presence of RbfA, a portion of the 16S rRNA encompassing helix 44, which is known to be directly involved in mRNA decoding and tRNA binding, is displaced. These results shed light on the role played by RbfA during maturation of the 30S subunit, and also indicate how RbfA provides cells with a translational advantage under conditions of cold shock.
Structural aspects of RbfA action during small ribosomal subunit assembly.,Datta PP, Wilson DN, Kawazoe M, Swami NK, Kaminishi T, Sharma MR, Booth TM, Takemoto C, Fucini P, Yokoyama S, Agrawal RK Mol Cell. 2007 Nov 9;28(3):434-45. PMID:17996707[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Datta PP, Wilson DN, Kawazoe M, Swami NK, Kaminishi T, Sharma MR, Booth TM, Takemoto C, Fucini P, Yokoyama S, Agrawal RK. Structural aspects of RbfA action during small ribosomal subunit assembly. Mol Cell. 2007 Nov 9;28(3):434-45. PMID:17996707 doi:10.1016/j.molcel.2007.08.026
- ↑ Datta PP, Wilson DN, Kawazoe M, Swami NK, Kaminishi T, Sharma MR, Booth TM, Takemoto C, Fucini P, Yokoyama S, Agrawal RK. Structural aspects of RbfA action during small ribosomal subunit assembly. Mol Cell. 2007 Nov 9;28(3):434-45. PMID:17996707 doi:10.1016/j.molcel.2007.08.026
|
