6gq1

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(New page: '''Unreleased structure''' The entry 6gq1 is ON HOLD Authors: Pellegrino, S., Demeshkina, N., Mancera-Martinez, E., Melnikov, S., Simonetti, A., Myasnikov, A., Yusupov, M., Yusupova, G....)
Current revision (16:33, 9 July 2025) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 6gq1 is ON HOLD
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==Cryo-EM reconstruction of yeast 80S ribosome in complex with mRNA, tRNA and eEF2 (GMPPCP/sordarin)==
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<SX load='6gq1' size='340' side='right' viewer='molstar' caption='[[6gq1]], [[Resolution|resolution]] 4.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[6gq1]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GQ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GQ1 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DDE:{3-[4-(2-AMINO-2-CARBOXY-ETHYL)-1H-IMIDAZOL-2-YL]-1-CARBAMOYL-PROPYL}-TRIMETHYL-AMMONIUM'>DDE</scene>, <scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>, <scene name='pdbligand=SO1:(1S,3R,3aR,4S,4aR,7R,7aR,8aS)-8a-{[(6-deoxy-4-O-methyl-beta-D-altropyranosyl)oxy]methyl}-4-formyl-7-methyl-3-(1-methylethyl)decahydro-1,4-methano-s-indacene-3a(1H)-carboxylic+acid'>SO1</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6gq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gq1 OCA], [https://pdbe.org/6gq1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6gq1 RCSB], [https://www.ebi.ac.uk/pdbsum/6gq1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6gq1 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RSSA1_YEAST RSSA1_YEAST] Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits.<ref>PMID:9973221</ref> <ref>PMID:14627813</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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One of the most critical steps of protein biosynthesis is the coupled movement of mRNA, which encodes genetic information, with tRNAs on the ribosome. In eukaryotes, this process is catalyzed by a conserved G-protein, the elongation factor 2 (eEF2), which carries a unique post-translational modification, called diphthamide, found in all eukaryotic species. Here we present near-atomic resolution cryo-electron microscopy structures of yeast 80S ribosome complexes containing mRNA, tRNA and eEF2 trapped in different GTP-hydrolysis states which provide further structural insights into the role of diphthamide in the mechanism of translation fidelity in eukaryotes.
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Authors: Pellegrino, S., Demeshkina, N., Mancera-Martinez, E., Melnikov, S., Simonetti, A., Myasnikov, A., Yusupov, M., Yusupova, G., Hashem, Y.
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Structural Insights into the Role of Diphthamide on Elongation Factor 2 in mRNA Reading-Frame Maintenance.,Pellegrino S, Demeshkina N, Mancera-Martinez E, Melnikov S, Simonetti A, Myasnikov A, Yusupov M, Yusupova G, Hashem Y J Mol Biol. 2018 Jun 7. pii: S0022-2836(18)30582-5. doi:, 10.1016/j.jmb.2018.06.006. PMID:29886014<ref>PMID:29886014</ref>
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Description: Cryo-EM reconstruction of yeast 80S ribosome in complex with mRNA, tRNA and eEF2 (GMPPCP/sordarin)
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Myasnikov, A]]
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<div class="pdbe-citations 6gq1" style="background-color:#fffaf0;"></div>
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[[Category: Pellegrino, S]]
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[[Category: Mancera-Martinez, E]]
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==See Also==
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[[Category: Yusupova, G]]
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*[[Elongation factor 3D structures|Elongation factor 3D structures]]
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[[Category: Hashem, Y]]
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*[[Ribosome 3D structures|Ribosome 3D structures]]
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[[Category: Yusupov, M]]
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== References ==
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[[Category: Demeshkina, N]]
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<references/>
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[[Category: Simonetti, A]]
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__TOC__
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[[Category: Melnikov, S]]
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</SX>
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[[Category: Large Structures]]
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[[Category: Saccharomyces cerevisiae S288C]]
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[[Category: Demeshkina N]]
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[[Category: Hashem Y]]
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[[Category: Mancera-Martinez E]]
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[[Category: Melnikov S]]
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[[Category: Myasnikov A]]
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[[Category: Pellegrino S]]
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[[Category: Simonetti A]]
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[[Category: Yusupov M]]
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[[Category: Yusupova G]]

Current revision

Cryo-EM reconstruction of yeast 80S ribosome in complex with mRNA, tRNA and eEF2 (GMPPCP/sordarin)

6gq1, resolution 4.40Å

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