2fbn

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==Plasmodium falciparum putative FK506-binding protein PFL2275c, C-terminal TPR-containing domain==
==Plasmodium falciparum putative FK506-binding protein PFL2275c, C-terminal TPR-containing domain==
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<StructureSection load='2fbn' size='340' side='right' caption='[[2fbn]], [[Resolution|resolution]] 1.63&Aring;' scene=''>
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<StructureSection load='2fbn' size='340' side='right'caption='[[2fbn]], [[Resolution|resolution]] 1.63&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[2fbn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Plafa Plafa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FBN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FBN FirstGlance]. <br>
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<table><tr><td colspan='2'>[[2fbn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FBN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FBN FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fbn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fbn OCA], [http://pdbe.org/2fbn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fbn RCSB], [http://www.ebi.ac.uk/pdbsum/2fbn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2fbn ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.63&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fbn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fbn OCA], [https://pdbe.org/2fbn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fbn RCSB], [https://www.ebi.ac.uk/pdbsum/2fbn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fbn ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FKB35_PLAF7 FKB35_PLAF7] Has peptidylprolyl isomerase (PPIase) and co-chaperone activities (PubMed:15664653, PubMed:15850699). Assists protein folding by catalyzing the peptidyl conversion of cis and trans rotamers of the prolyl amide bond of protein substrates (PubMed:15664653, PubMed:15850699, PubMed:23974147). Inhibits calcineurin phosphatase activity in vitro (PubMed:15850699, PubMed:17289400, PubMed:23974147). Plays an essential role in merozoite egress from host erythrocytes (PubMed:15664653, PubMed:23974147).<ref>PMID:15664653</ref> <ref>PMID:15850699</ref> <ref>PMID:17289400</ref> <ref>PMID:23974147</ref>
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fbn ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fbn ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
 
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== Publication Abstract from PubMed ==
 
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Plasmodium falciparum FK506-binding protein 35 (PfFKBP35) that binds to FK506 contains a conserved tetratricopeptide repeat (TPR) domain. Several known TPR domains such as Hop, PPP5, CHIP, and FKBP52 are structurally conserved and are able to interact with molecular chaperones such as Hsp70/Hsp90. Here, we present the crystal structure of PfFKBP35-TPR and demonstrate its interaction with Hsp90 C-terminal pentapeptide (MEEVD) by surface plasmon resonance and nuclear magnetic resonance spectroscopy-based binding studies. Our sequence and structural analyses reveal that PfFKBP35 is similar to Hop and PPP5 in possessing all the conserved residues which are important for carboxylate clamping with Hsp90. Mutational studies were carried out on positively charged clamp residues that are crucial for binding to carboxylate groups of aspartate, showing that all the mutated residues are important for Hsp90 binding. Molecular docking and electrostatic calculations demonstrated that the MEEVD peptide of Hsp90 can form aspartate clamp unlike FKBP52. Our results provide insightful information and structural basis about the molecular interaction between PfFKBP35-TPR and Hsp90.
 
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Crystallographic structure of the tetratricopeptide repeat domain of Plasmodium falciparum FKBP35 and its molecular interaction with Hsp90 C-terminal pentapeptide.,Alag R, Bharatham N, Dong A, Hills T, Harikishore A, Widjaja AA, Shochat SG, Hui R, Yoon HS Protein Sci. 2009 Oct;18(10):2115-24. PMID:19691130<ref>PMID:19691130</ref>
 
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
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</div>
 
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<div class="pdbe-citations 2fbn" style="background-color:#fffaf0;"></div>
 
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Plafa]]
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[[Category: Large Structures]]
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[[Category: Arrowsmith, C H]]
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[[Category: Plasmodium falciparum]]
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[[Category: Bochkarev, A]]
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[[Category: Arrowsmith CH]]
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[[Category: Dong, A]]
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[[Category: Bochkarev A]]
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[[Category: Edwards, A M]]
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[[Category: Dong A]]
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[[Category: Hills, T]]
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[[Category: Edwards AM]]
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[[Category: Hui, R]]
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[[Category: Hills T]]
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[[Category: Koeieradzki, I]]
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[[Category: Hui R]]
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[[Category: Lew, J]]
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[[Category: Koeieradzki I]]
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[[Category: Melone, M]]
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[[Category: Lew J]]
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[[Category: Structural genomic]]
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[[Category: Melone M]]
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[[Category: Sundararajan, E]]
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[[Category: Sundararajan E]]
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[[Category: Sundstrom, M]]
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[[Category: Sundstrom M]]
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[[Category: Wasney, G]]
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[[Category: Wasney G]]
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[[Category: Weigelt, J]]
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[[Category: Weigelt J]]
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[[Category: Zhao, Y]]
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[[Category: Zhao Y]]
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[[Category: Pfl2275c]]
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[[Category: Sgc]]
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[[Category: Sulfur sad]]
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[[Category: Tpr-containing domain]]
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[[Category: Unknown function]]
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Current revision

Plasmodium falciparum putative FK506-binding protein PFL2275c, C-terminal TPR-containing domain

PDB ID 2fbn

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