2jfs

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[[Image:2jfs.jpg|left|200px]]
 
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{{Structure
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==Crystal structure of the PPM Ser-Thr phosphatase MsPP from Mycobacterium smegmatis in complex with cacodylate==
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|PDB= 2jfs |SIZE=350|CAPTION= <scene name='initialview01'>2jfs</scene>, resolution 1.45&Aring;
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<StructureSection load='2jfs' size='340' side='right'caption='[[2jfs]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
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|SITE= <scene name='pdbsite=AC1:Gol+Binding+Site+For+Chain+A'>AC1</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>
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<table><tr><td colspan='2'>[[2jfs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JFS FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jfs OCA], [https://pdbe.org/2jfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jfs RCSB], [https://www.ebi.ac.uk/pdbsum/2jfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jfs ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jfs OCA], [http://www.ebi.ac.uk/pdbsum/2jfs PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2jfs RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/A0QTQ6_MYCS2 A0QTQ6_MYCS2]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jf/2jfs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jfs ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Serine/threonine-specific phosphatases (PPs) represent, after protein tyrosine phosphatases, the second major class of enzymes that catalyze the dephosphorylation of proteins. They are classed in two large families, known as PPP and PPM, on the basis of sequence similarities, metal ion dependence, and inhibitor sensitivity. Despite their wide species distribution and broad physiological roles, the catalytic mechanism of PPM phosphatases has been primarily inferred from studies of a single enzyme, human PP2Calpha. Here, we report the biochemical characterization and the atomic resolution structures of a soluble PPM phosphatase from the saprophyte Mycobacterium smegmatis in complex with different ligands. The structures provide putative snapshots along the catalytic cycle, which support an associative reaction mechanism that differs in some important aspects from the currently accepted model and reinforces the hypothesis of convergent evolution in PPs.
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'''CRYSTAL STRUCTURE OF THE PPM SER-THR PHOSPHATASE MSPP FROM MYCOBACTERIUM SMEGMATIS IN COMPLEX WITH CACODYLATE'''
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Insights into the catalytic mechanism of PPM Ser/Thr phosphatases from the atomic resolution structures of a mycobacterial enzyme.,Bellinzoni M, Wehenkel A, Shepard W, Alzari PM Structure. 2007 Jul;15(7):863-72. PMID:17637345<ref>PMID:17637345</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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2JFS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis Mycobacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JFS OCA].
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<div class="pdbe-citations 2jfs" style="background-color:#fffaf0;"></div>
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[[Category: Mycobacterium smegmatis]]
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== References ==
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[[Category: Single protein]]
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<references/>
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[[Category: Alzari, P M.]]
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__TOC__
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[[Category: Bellinzoni, M.]]
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</StructureSection>
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[[Category: Shepard, W.]]
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[[Category: Large Structures]]
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[[Category: Wehenkel, A.]]
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[[Category: Mycolicibacterium smegmatis MC2 155]]
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[[Category: cacodylate]]
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[[Category: Alzari PM]]
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[[Category: hydrolase]]
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[[Category: Bellinzoni M]]
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[[Category: manganese]]
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[[Category: Shepard W]]
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[[Category: mycobacterium]]
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[[Category: Wehenkel A]]
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[[Category: ppm phosphatase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:57:35 2008''
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Current revision

Crystal structure of the PPM Ser-Thr phosphatase MsPP from Mycobacterium smegmatis in complex with cacodylate

PDB ID 2jfs

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