6gsz
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6gsz is ON HOLD Authors: Pachl, P., Rezacova, P., Skerlova, J. Description: Crystal structure of native alfa-L-rhamnosidase from Aspergillus terreu...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of native alfa-L-rhamnosidase from Aspergillus terreus== | |
| + | <StructureSection load='6gsz' size='340' side='right'caption='[[6gsz]], [[Resolution|resolution]] 1.38Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6gsz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_terreus Aspergillus terreus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GSZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GSZ FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.38Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DTU:(2R,3S)-1,4-DIMERCAPTOBUTANE-2,3-DIOL'>DTU</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MPO:3[N-MORPHOLINO]PROPANE+SULFONIC+ACID'>MPO</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6gsz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gsz OCA], [https://pdbe.org/6gsz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6gsz RCSB], [https://www.ebi.ac.uk/pdbsum/6gsz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6gsz ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/I0AZ41_ASPTE I0AZ41_ASPTE] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | alpha-L-Rhamnosidases cleave terminal nonreducing alpha-L-rhamnosyl residues from many natural rhamnoglycosides. This makes them catalysts of interest for various biotechnological applications. The X-ray structure of the GH78 family alpha-L-rhamnosidase from Aspergillus terreus has been determined at 1.38 A resolution using the sulfur single-wavelength anomalous dispersion phasing method. The protein was isolated from its natural source in the native glycosylated form, and the active site contained a glucose molecule, probably from the growth medium. In addition to its catalytic domain, the alpha-L-rhamnosidase from A. terreus contains four accessory domains of unknown function. The structural data suggest that two of these accessory domains, E and F, might play a role in stabilizing the aglycon portion of the bound substrate. | ||
| - | + | Crystal structure of native alpha-L-rhamnosidase from Aspergillus terreus.,Pachl P, Skerlova J, Simcikova D, Kotik M, Krenkova A, Mader P, Brynda J, Kapesova J, Kren V, Otwinowski Z, Rezacova P Acta Crystallogr D Struct Biol. 2018 Nov 1;74(Pt 11):1078-1084. doi:, 10.1107/S2059798318013049. Epub 2018 Oct 29. PMID:30387766<ref>PMID:30387766</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6gsz" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: Skerlova | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Aspergillus terreus]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Pachl P]] | ||
| + | [[Category: Rezacova P]] | ||
| + | [[Category: Skerlova J]] | ||
Current revision
Crystal structure of native alfa-L-rhamnosidase from Aspergillus terreus
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