6ccd

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==The crystal structure of Mycobacterium tuberculosis Rv1747 FHA-1==
==The crystal structure of Mycobacterium tuberculosis Rv1747 FHA-1==
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<StructureSection load='6ccd' size='340' side='right' caption='[[6ccd]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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<StructureSection load='6ccd' size='340' side='right'caption='[[6ccd]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6ccd]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CCD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CCD FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6ccd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CCD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CCD FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ccd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ccd OCA], [http://pdbe.org/6ccd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ccd RCSB], [http://www.ebi.ac.uk/pdbsum/6ccd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ccd ProSAT]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ccd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ccd OCA], [https://pdbe.org/6ccd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ccd RCSB], [https://www.ebi.ac.uk/pdbsum/6ccd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ccd ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/ABC1_MYCTU ABC1_MYCTU]] Involved in the translocation of an unknown substrate across the membrane. Transmembrane domains (TMD) form a pore in the membrane and the ATP-binding domain (NBD) is responsible for energy generation. Required for virulence.<ref>PMID:15135525</ref> <ref>PMID:16040957</ref>
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[https://www.uniprot.org/uniprot/ABC1_MYCTU ABC1_MYCTU] Involved in the translocation of an unknown substrate across the membrane. Transmembrane domains (TMD) form a pore in the membrane and the ATP-binding domain (NBD) is responsible for energy generation. Required for virulence.<ref>PMID:15135525</ref> <ref>PMID:16040957</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Mycobacterium tuberculosis ATP-binding cassette transporter Rv1747 is a putative exporter of cell wall biosynthesis intermediates. Rv1747 has a cytoplasmic regulatory module consisting of two pThr-interacting Forkhead-associated (FHA) domains connected by a conformationally disordered linker with two phospho-acceptor threonines (pThr). The structures of FHA-1 and FHA-2 were determined by X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy, respectively. Relative to the canonical 11-strand beta-sandwich FHA domain fold of FHA-1, FHA-2 is circularly permuted and lacking one beta-strand. Nevertheless, the two share a conserved pThr-binding cleft. FHA-2 is less stable and more dynamic than FHA-1, yet binds model pThr peptides with moderately higher affinity ( approximately 50 muM versus 500 muM equilibrium dissociation constants). Based on NMR relaxation and chemical shift perturbation measurements, when joined within a polypeptide chain, either FHA domain can bind either linker pThr to form intra- and intermolecular complexes. We hypothesize that this enables tunable phosphorylation-dependent multimerization to regulate Rv1747 transporter activity.
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Biophysical Characterization of the Tandem FHA Domain Regulatory Module from the Mycobacteriumtuberculosis ABC Transporter Rv1747.,Heinkel F, Shen L, Richard-Greenblatt M, Okon M, Bui JM, Gee CL, Gay LM, Alber T, Av-Gay Y, Gsponer J, McIntosh LP Structure. 2018 May 17. pii: S0969-2126(18)30164-3. doi:, 10.1016/j.str.2018.04.018. PMID:29861345<ref>PMID:29861345</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6ccd" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Alber, T]]
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[[Category: Large Structures]]
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[[Category: Gay, L M]]
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[[Category: Mycobacterium tuberculosis H37Rv]]
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[[Category: Gee, C L]]
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[[Category: Alber T]]
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[[Category: Fha domain]]
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[[Category: Gay LM]]
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[[Category: Phospho-peptide binding domain]]
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[[Category: Gee CL]]
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[[Category: Phospho-threonine binding domain]]
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[[Category: Protein binding]]
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Current revision

The crystal structure of Mycobacterium tuberculosis Rv1747 FHA-1

PDB ID 6ccd

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