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6dub

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(New page: '''Unreleased structure''' The entry 6dub is ON HOLD Authors: Dong, C., Tempel, W., Li, Y., Bountra, C., Arrowsmith, C.H., Edwards, A.M., Min, J., Structural Genomics Consortium (SGC) ...)
Current revision (06:14, 11 October 2023) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 6dub is ON HOLD
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==Crystal structure of a methyltransferase==
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<StructureSection load='6dub' size='340' side='right'caption='[[6dub]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[6dub]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DUB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DUB FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5JP:N-METHYL-L-SERINE'>5JP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6dub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dub OCA], [https://pdbe.org/6dub PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6dub RCSB], [https://www.ebi.ac.uk/pdbsum/6dub PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6dub ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NTM1B_HUMAN NTM1B_HUMAN] Alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes monomethylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and Pro in the Pro-Pro-Lys motif. May activate NTMT1 by priming its substrates for trimethylation.<ref>PMID:24090352</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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alpha-N-terminal methylation of proteins is an important post-translational modification that is catalyzed by two different N-terminal methyltransferases, namely NTMT1 and NTMT2. Previous studies have suggested that NTMT1 is a tri-methyltransferase, whereas NTMT2 is a mono-methyltransferase. Here, we report the first crystal structures, to our knowledge, of NTMT2 in binary complex with S-adenosyl-L-methionine as well as in ternary complex with S-adenosyl-L-homocysteine and a substrate peptide. Our structural observations combined with biochemical studies reveal that NTMT2 is also able to di-/tri-methylate the GPKRIA peptide and di-methylate the PPKRIA peptide, otherwise it is predominantly a mono-methyltransferase. The residue N89 of NTMT2 serves as a gatekeeper residue that regulates the binding of unmethylated versus monomethylated substrate peptide. Structural comparison of NTMT1 and NTMT2 prompts us to design a N89G mutant of NTMT2 that can profoundly alter its catalytic activities and product specificities.
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Authors: Dong, C., Tempel, W., Li, Y., Bountra, C., Arrowsmith, C.H., Edwards, A.M., Min, J., Structural Genomics Consortium (SGC)
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An asparagine/glycine switch governs product specificity of human N-terminal methyltransferase NTMT2.,Dong C, Dong G, Li L, Zhu L, Tempel W, Liu Y, Huang R, Min J Commun Biol. 2018 Nov 2;1:183. doi: 10.1038/s42003-018-0196-2. eCollection 2018. PMID:30417120<ref>PMID:30417120</ref>
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Description: Crystal structure of a methyltransferase
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Min, J]]
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<div class="pdbe-citations 6dub" style="background-color:#fffaf0;"></div>
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[[Category: Edwards, A.M]]
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== References ==
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[[Category: Arrowsmith, C.H]]
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<references/>
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[[Category: Structural Genomics Consortium (Sgc)]]
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__TOC__
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[[Category: Dong, C]]
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</StructureSection>
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[[Category: Li, Y]]
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[[Category: Homo sapiens]]
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[[Category: Bountra, C]]
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[[Category: Large Structures]]
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[[Category: Tempel, W]]
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[[Category: Arrowsmith CH]]
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[[Category: Bountra C]]
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[[Category: Dong C]]
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[[Category: Edwards AM]]
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[[Category: Li Y]]
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[[Category: Min J]]
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[[Category: Tempel W]]

Current revision

Crystal structure of a methyltransferase

PDB ID 6dub

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