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| | ==NMR structure for the CgNa toxin from the sea anemone Condylactis gigantea== | | ==NMR structure for the CgNa toxin from the sea anemone Condylactis gigantea== |
| - | <StructureSection load='2h9x' size='340' side='right' caption='[[2h9x]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2h9x' size='340' side='right'caption='[[2h9x]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2h9x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Condylactis_gigantea Condylactis gigantea]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H9X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2H9X FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2h9x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Condylactis_gigantea Condylactis gigantea]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H9X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2H9X FirstGlance]. <br> |
| | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene></td></tr> | | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h9x OCA], [http://pdbe.org/2h9x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2h9x RCSB], [http://www.ebi.ac.uk/pdbsum/2h9x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2h9x ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2h9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h9x OCA], [https://pdbe.org/2h9x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2h9x RCSB], [https://www.ebi.ac.uk/pdbsum/2h9x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2h9x ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TXNA_CONGI TXNA_CONGI]] Slows down the inactivation process of TTX-sensitive voltage-gated sodium channels (Nav). Has a strong crab-paralyzing activity.<ref>PMID:16814340</ref> | + | [[https://www.uniprot.org/uniprot/TXNA_CONGI TXNA_CONGI]] Slows down the inactivation process of TTX-sensitive voltage-gated sodium channels (Nav). Has a strong crab-paralyzing activity.<ref>PMID:16814340</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Condylactis gigantea]] | | [[Category: Condylactis gigantea]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Gimenez-Gallego, G]] | | [[Category: Gimenez-Gallego, G]] |
| | [[Category: Jimenez-Barbero, J]] | | [[Category: Jimenez-Barbero, J]] |
| Structural highlights
Function
[TXNA_CONGI] Slows down the inactivation process of TTX-sensitive voltage-gated sodium channels (Nav). Has a strong crab-paralyzing activity.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
CgNa (Condylactis gigantea neurotoxin) is a 47-amino-acid- residue toxin from the giant Caribbean sea anemone Condylactis gigantea. The structure of CgNa, which was solved by 1H-NMR spectroscopy, is somewhat atypical and displays significant homology with both type I and II anemone toxins. CgNa also displays a considerable number of exceptions to the canonical structural elements that are thought to be essential for the activity of this group of toxins. Furthermore, unique residues in CgNa define a characteristic structure with strong negatively charged surface patches. These patches disrupt a surface-exposed cluster of hydrophobic residues present in all anemone-derived toxins described to date. A thorough characterization by patch-clamp analysis using rat DRG (dorsal root ganglion) neurons indicated that CgNa preferentially binds to TTX-S (tetrodotoxin-sensitive) voltage-gated sodium channels in the resting state. This association increased the inactivation time constant and the rate of recovery from inactivation, inducing a significant shift in the steady state of inactivation curve to the left. The specific structural features of CgNa may explain its weaker inhibitory capacity when compared with the other type I and II anemone toxins.
CgNa, a type I toxin from the giant Caribbean sea anemone Condylactis gigantea shows structural similarities to both type I and II toxins, as well as distinctive structural and functional properties(1).,Salceda E, Perez-Castells J, Lopez-Mendez B, Garateix A, Salazar H, Lopez O, Aneiros A, Standker L, Beress L, Forssmann WG, Soto E, Jimenez-Barbero J, Gimenez-Gallego G Biochem J. 2007 Aug 15;406(1):67-76. PMID:17506725[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Standker L, Beress L, Garateix A, Christ T, Ravens U, Salceda E, Soto E, John H, Forssmann WG, Aneiros A. A new toxin from the sea anemone Condylactis gigantea with effect on sodium channel inactivation. Toxicon. 2006 Aug;48(2):211-20. Epub 2006 May 19. PMID:16814340 doi:10.1016/j.toxicon.2006.05.001
- ↑ Salceda E, Perez-Castells J, Lopez-Mendez B, Garateix A, Salazar H, Lopez O, Aneiros A, Standker L, Beress L, Forssmann WG, Soto E, Jimenez-Barbero J, Gimenez-Gallego G. CgNa, a type I toxin from the giant Caribbean sea anemone Condylactis gigantea shows structural similarities to both type I and II toxins, as well as distinctive structural and functional properties(1). Biochem J. 2007 Aug 15;406(1):67-76. PMID:17506725 doi:10.1042/BJ20070130
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