6gxt
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6gxt is ON HOLD Authors: Schulz, E.C., Mehrabi, P., Mueller-Werkmeister, H., Tellkamp, F., Stuart, W., Persch, E., De Gasparo, R., Diederich, F., Pa...) |
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| - | '''Unreleased structure''' | ||
| - | The | + | ==The hit-and-return system enables efficient time-resolved serial synchrotron crystallography: FAcD2052MS after reaction initiation== |
| + | <StructureSection load='6gxt' size='340' side='right' caption='[[6gxt]], [[Resolution|resolution]] 1.95Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6gxt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhopa Rhopa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GXT FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAH:FLUOROACETIC+ACID'>FAH</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RPA1163 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=258594 RHOPA])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Haloacetate_dehalogenase Haloacetate dehalogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.3 3.8.1.3] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gxt OCA], [http://pdbe.org/6gxt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gxt RCSB], [http://www.ebi.ac.uk/pdbsum/6gxt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gxt ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/DEHA_RHOPA DEHA_RHOPA]] Catalyzes the hydrolytic defluorination of fluoroacetate to produce glycolate. Has lower activity towards bromoacetate and chloroacetate.<ref>PMID:21510690</ref> <ref>PMID:21510690</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | We present a 'hit-and-return' (HARE) method for time-resolved serial synchrotron crystallography with time resolution from milliseconds to seconds or longer. Timing delays are set mechanically, using the regular pattern in fixed-target crystallography chips and a translation stage system. Optical pump-probe experiments to capture intermediate structures of fluoroacetate dehalogenase binding to its ligand demonstrated that data can be collected at short (30 ms), medium (752 ms) and long (2,052 ms) intervals. | ||
| - | + | The hit-and-return system enables efficient time-resolved serial synchrotron crystallography.,Schulz EC, Mehrabi P, Muller-Werkmeister HM, Tellkamp F, Jha A, Stuart W, Persch E, De Gasparo R, Diederich F, Pai EF, Miller RJD Nat Methods. 2018 Nov;15(11):901-904. doi: 10.1038/s41592-018-0180-2. Epub 2018, Oct 30. PMID:30377366<ref>PMID:30377366</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6gxt" style="background-color:#fffaf0;"></div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Haloacetate dehalogenase]] | ||
| + | [[Category: Rhopa]] | ||
| + | [[Category: Diederich, F]] | ||
| + | [[Category: Gasparo, R De]] | ||
[[Category: Mehrabi, P]] | [[Category: Mehrabi, P]] | ||
| - | + | [[Category: Miller, R J.D]] | |
| - | + | ||
| - | + | ||
| - | [[Category: Miller, R | + | |
[[Category: Mueller-Werkmeister, H]] | [[Category: Mueller-Werkmeister, H]] | ||
| - | [[Category: | + | [[Category: Pai, E F]] |
[[Category: Persch, E]] | [[Category: Persch, E]] | ||
| - | [[Category: Schulz, E | + | [[Category: Schulz, E C]] |
| + | [[Category: Stuart, W]] | ||
| + | [[Category: Tellkamp, F]] | ||
| + | [[Category: Dna binding protein]] | ||
| + | [[Category: Pump-probe experiment]] | ||
| + | [[Category: Structural enzymology]] | ||
| + | [[Category: Time-resolved crystallography]] | ||
Current revision
The hit-and-return system enables efficient time-resolved serial synchrotron crystallography: FAcD2052MS after reaction initiation
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