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| | ==Crystal structure of the SYCP1 N-terminal head-to-head assembly in closed conformation== | | ==Crystal structure of the SYCP1 N-terminal head-to-head assembly in closed conformation== |
| - | <StructureSection load='6f5x' size='340' side='right' caption='[[6f5x]], [[Resolution|resolution]] 1.91Å' scene=''> | + | <StructureSection load='6f5x' size='340' side='right'caption='[[6f5x]], [[Resolution|resolution]] 1.91Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6f5x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F5X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6F5X FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6f5x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F5X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6F5X FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.91Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SYCP1, SCP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6f5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f5x OCA], [http://pdbe.org/6f5x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6f5x RCSB], [http://www.ebi.ac.uk/pdbsum/6f5x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6f5x ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6f5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f5x OCA], [https://pdbe.org/6f5x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6f5x RCSB], [https://www.ebi.ac.uk/pdbsum/6f5x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6f5x ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SYCP1_HUMAN SYCP1_HUMAN]] Major component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Required for normal assembly of the central element of the synaptonemal complexes. Required for normal centromere pairing during meiosis. Required for normal meiotic chromosome synapsis during oocyte and spermatocyte development and for normal male and female fertility.[UniProtKB:Q62209] | + | [https://www.uniprot.org/uniprot/SYCP1_HUMAN SYCP1_HUMAN] Major component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Required for normal assembly of the central element of the synaptonemal complexes. Required for normal centromere pairing during meiosis. Required for normal meiotic chromosome synapsis during oocyte and spermatocyte development and for normal male and female fertility.[UniProtKB:Q62209] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Davies, O R]] | + | [[Category: Large Structures]] |
| - | [[Category: Dunce, J M]] | + | [[Category: Davies OR]] |
| - | [[Category: Ratcliff, M]] | + | [[Category: Dunce JM]] |
| - | [[Category: Chromosome structure]] | + | [[Category: Ratcliff M]] |
| - | [[Category: Coiled-coil]]
| + | |
| - | [[Category: Meiosis]]
| + | |
| - | [[Category: Self-assembly]]
| + | |
| - | [[Category: Structural protein]]
| + | |
| Structural highlights
Function
SYCP1_HUMAN Major component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Required for normal assembly of the central element of the synaptonemal complexes. Required for normal centromere pairing during meiosis. Required for normal meiotic chromosome synapsis during oocyte and spermatocyte development and for normal male and female fertility.[UniProtKB:Q62209]
Publication Abstract from PubMed
Meiotic chromosomes adopt unique structures in which linear arrays of chromatin loops are bound together in homologous chromosome pairs by a supramolecular protein assembly, the synaptonemal complex. This three-dimensional scaffold provides the essential structural framework for genetic exchange by crossing over and subsequent homolog segregation. The core architecture of the synaptonemal complex is provided by SYCP1. Here we report the structure and self-assembly mechanism of human SYCP1 through X-ray crystallographic and biophysical studies. SYCP1 has an obligate tetrameric structure in which an N-terminal four-helical bundle bifurcates into two elongated C-terminal dimeric coiled-coils. This building block assembles into a zipper-like lattice through two self-assembly sites. N-terminal sites undergo cooperative head-to-head assembly in the midline, while C-terminal sites interact back to back on the chromosome axis. Our work reveals the underlying molecular structure of the synaptonemal complex in which SYCP1 self-assembly generates a supramolecular lattice that mediates meiotic chromosome synapsis.
Structural basis of meiotic chromosome synapsis through SYCP1 self-assembly.,Dunce JM, Dunne OM, Ratcliff M, Millan C, Madgwick S, Uson I, Davies OR Nat Struct Mol Biol. 2018 Jun 18. pii: 10.1038/s41594-018-0078-9. doi:, 10.1038/s41594-018-0078-9. PMID:29915389[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Dunce JM, Dunne OM, Ratcliff M, Millan C, Madgwick S, Uson I, Davies OR. Structural basis of meiotic chromosome synapsis through SYCP1 self-assembly. Nat Struct Mol Biol. 2018 Jun 18. pii: 10.1038/s41594-018-0078-9. doi:, 10.1038/s41594-018-0078-9. PMID:29915389 doi:http://dx.doi.org/10.1038/s41594-018-0078-9
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