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2npr

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[[Image:2npr.gif|left|200px]]
 
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{{Structure
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==Structural Studies on Plasmodium vivax Merozoite Surface Protein-1==
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|PDB= 2npr |SIZE=350|CAPTION= <scene name='initialview01'>2npr</scene>
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<StructureSection load='2npr' size='340' side='right'caption='[[2npr]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[2npr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_vivax_strain_Belem Plasmodium vivax strain Belem]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NPR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NPR FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE= Msp1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5855 Plasmodium vivax])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2npr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2npr OCA], [https://pdbe.org/2npr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2npr RCSB], [https://www.ebi.ac.uk/pdbsum/2npr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2npr ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2npr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2npr OCA], [http://www.ebi.ac.uk/pdbsum/2npr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2npr RCSB]</span>
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[https://www.uniprot.org/uniprot/Q8I1L1_PLAVI Q8I1L1_PLAVI]
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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'''Structural Studies on Plasmodium vivax Merozoite Surface Protein-1'''
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/np/2npr_consurf.spt"</scriptWhenChecked>
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==Overview==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2npr ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
Plasmodium vivax infection is the second most common cause of malaria throughout the world. Like other Plasmodium species, P. vivax has a large protein complex, MSP-1, located on the merozoite surface. The C-terminal MSP-1 sub-unit, MSP-1(42), is cleaved during red blood cell invasion, causing the majority of the complex to be shed and leaving only a small 15kDa sub-unit, MSP-1(19), on the merozite surface. MSP-1(19) is considered a strong vaccine candidate. We have determined the solution structure of MSP-1(19) from P. vivax using nuclear magnetic resonance (NMR) and show that, like in other Plasmodium species, it consists of two EGF-like domains that are oriented head-to-tail. The protein has a flat, disk-like shape with a highly charged surface. When MSP-1(19) is part of the larger MSP-1(42) precursor it exists as an independent domain with no stable contacts to the rest of the sub-unit. Gel filtration and analytical ultracentrifugation experiments indicate that P. vivax MSP-1(42) exists as a dimer in solution. MSP-1(19) itself is a monomer, however, 35 amino-acids immediately upstream of its N-terminus are sufficient to cause dimerization. Our data suggest that if MSP-1(42) exists as a dimer in vivo, secondary processing would cause the dissociation of two tightly linked MSP-1(19) proteins on the merozoite surface just prior to invasion.
Plasmodium vivax infection is the second most common cause of malaria throughout the world. Like other Plasmodium species, P. vivax has a large protein complex, MSP-1, located on the merozoite surface. The C-terminal MSP-1 sub-unit, MSP-1(42), is cleaved during red blood cell invasion, causing the majority of the complex to be shed and leaving only a small 15kDa sub-unit, MSP-1(19), on the merozite surface. MSP-1(19) is considered a strong vaccine candidate. We have determined the solution structure of MSP-1(19) from P. vivax using nuclear magnetic resonance (NMR) and show that, like in other Plasmodium species, it consists of two EGF-like domains that are oriented head-to-tail. The protein has a flat, disk-like shape with a highly charged surface. When MSP-1(19) is part of the larger MSP-1(42) precursor it exists as an independent domain with no stable contacts to the rest of the sub-unit. Gel filtration and analytical ultracentrifugation experiments indicate that P. vivax MSP-1(42) exists as a dimer in solution. MSP-1(19) itself is a monomer, however, 35 amino-acids immediately upstream of its N-terminus are sufficient to cause dimerization. Our data suggest that if MSP-1(42) exists as a dimer in vivo, secondary processing would cause the dissociation of two tightly linked MSP-1(19) proteins on the merozoite surface just prior to invasion.
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==About this Structure==
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Structural studies on Plasmodium vivax merozoite surface protein-1.,Babon JJ, Morgan WD, Kelly G, Eccleston JF, Feeney J, Holder AA Mol Biochem Parasitol. 2007 May;153(1):31-40. Epub 2007 Jan 30. PMID:17343930<ref>PMID:17343930</ref>
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2NPR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_vivax Plasmodium vivax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NPR OCA].
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==Reference==
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Structural studies on Plasmodium vivax merozoite surface protein-1., Babon JJ, Morgan WD, Kelly G, Eccleston JF, Feeney J, Holder AA, Mol Biochem Parasitol. 2007 May;153(1):31-40. Epub 2007 Jan 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17343930 17343930]
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[[Category: Plasmodium vivax]]
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[[Category: Single protein]]
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[[Category: Babon, J J.]]
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[[Category: Eccleston, J F.]]
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[[Category: Feeney, J.]]
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[[Category: Holder, A A.]]
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[[Category: Kelly, G.]]
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[[Category: Morgan, W D.]]
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[[Category: egf-like domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:06:32 2008''
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2npr" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Plasmodium vivax strain Belem]]
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[[Category: Babon JJ]]
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[[Category: Eccleston JF]]
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[[Category: Feeney J]]
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[[Category: Holder AA]]
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[[Category: Kelly G]]
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[[Category: Morgan WD]]

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Structural Studies on Plasmodium vivax Merozoite Surface Protein-1

PDB ID 2npr

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