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| | ==Structure of Triosephosphate Isomerase of Tenebrio molitor== | | ==Structure of Triosephosphate Isomerase of Tenebrio molitor== |
| - | <StructureSection load='2i9e' size='340' side='right' caption='[[2i9e]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='2i9e' size='340' side='right'caption='[[2i9e]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2i9e]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Tenmo Tenmo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I9E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I9E FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2i9e]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Tenebrio_molitor Tenebrio molitor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I9E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I9E FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tpi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7067 TENMO])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i9e OCA], [https://pdbe.org/2i9e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i9e RCSB], [https://www.ebi.ac.uk/pdbsum/2i9e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i9e ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i9e OCA], [http://pdbe.org/2i9e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i9e RCSB], [http://www.ebi.ac.uk/pdbsum/2i9e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2i9e ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8MPF2_TENMO Q8MPF2_TENMO] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Tenmo]] | + | [[Category: Large Structures]] |
| - | [[Category: Triose-phosphate isomerase]] | + | [[Category: Tenebrio molitor]] |
| - | [[Category: Hoehne, W]] | + | [[Category: Hoehne W]] |
| - | [[Category: Krauss, N]] | + | [[Category: Krauss N]] |
| - | [[Category: Scheerer, P]] | + | [[Category: Scheerer P]] |
| - | [[Category: Schmidt, A]] | + | [[Category: Schmidt A]] |
| - | [[Category: Wessner, H]] | + | [[Category: Wessner H]] |
| - | [[Category: Isomerase]]
| + | |
| - | [[Category: Triosephosphate isomerase tenebrio molitor]]
| + | |
| Structural highlights
Function
Q8MPF2_TENMO
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
A coleopteran triosephosphate isomerase (TIM) from Tenebrio molitor (yellow mealworm beetle) was recombinantly expressed in Escherichia coli and characterized with respect to thermal stability, kinetic parameters and oligomeric state. The enzyme was successfully crystallized and the structure determined by X-ray analysis to 2.0 A resolution. This is the first example of an invertebrate TIM. We compare structural features with known structures of TIMs from microorganisms, plants and vertebrates, and discuss the utility of the Tenebrio TIM sequence, together with several newly sequenced insect TIMs, for molecular phylogenetic analysis.
A coleopteran triosephosphate isomerase: X-ray structure and phylogenetic impact of insect sequences.,Knobeloch D, Schmidt A, Scheerer P, Krauss N, Wessner H, Scholz Ch, Kuttner G, von Rintelen T, Wessel A, Hohne W Insect Mol Biol. 2010 Feb;19(1):35-48. Epub 2009 Oct 22. PMID:19849721[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Knobeloch D, Schmidt A, Scheerer P, Krauss N, Wessner H, Scholz Ch, Kuttner G, von Rintelen T, Wessel A, Hohne W. A coleopteran triosephosphate isomerase: X-ray structure and phylogenetic impact of insect sequences. Insect Mol Biol. 2010 Feb;19(1):35-48. Epub 2009 Oct 22. PMID:19849721 doi:10.1111/j.1365-2583.2009.00928.x
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