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| | ==HUMAN MED26 N-TERMINAL DOMAIN (1-92)== | | ==HUMAN MED26 N-TERMINAL DOMAIN (1-92)== |
| - | <StructureSection load='5odd' size='340' side='right' caption='[[5odd]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='5odd' size='340' side='right'caption='[[5odd]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5odd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2mzo 2mzo]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ODD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ODD FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5odd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2mzo 2mzo]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ODD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ODD FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MED26, ARC70, CRSP7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5odd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5odd OCA], [https://pdbe.org/5odd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5odd RCSB], [https://www.ebi.ac.uk/pdbsum/5odd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5odd ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5odd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5odd OCA], [http://pdbe.org/5odd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5odd RCSB], [http://www.ebi.ac.uk/pdbsum/5odd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5odd ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MED26_HUMAN MED26_HUMAN]] Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. | + | [https://www.uniprot.org/uniprot/MED26_HUMAN MED26_HUMAN] Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 5odd" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 5odd" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Mediator|Mediator]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Cantrelle, F X]] | + | [[Category: Large Structures]] |
| - | [[Category: Dewitte, F]] | + | [[Category: Cantrelle F-X]] |
| - | [[Category: Hanoulle, X]] | + | [[Category: Dewitte F]] |
| - | [[Category: Landrieu, I]] | + | [[Category: Hanoulle X]] |
| - | [[Category: Lens, Z]] | + | [[Category: Landrieu I]] |
| - | [[Category: Perruzini, R]] | + | [[Category: Lens Z]] |
| - | [[Category: Verger, A]] | + | [[Category: Perruzini R]] |
| - | [[Category: Villeret, V]] | + | [[Category: Verger A]] |
| - | [[Category: Mediator complex transcriptional regulation]]
| + | [[Category: Villeret V]] |
| - | [[Category: Transcription]]
| + | |
| Structural highlights
Function
MED26_HUMAN Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors.
Publication Abstract from PubMed
MED26 is a subunit of the Mediator, a very large complex involved in regulation of gene transcription by RNA Polymerase II. MED26 regulates the switch between initiation and elongation phases of the transcription. This function requires interaction of its N-terminal domain (NTD) with several protein partners implicated in transcriptional regulation. Molecular details of the structure and interaction mode of MED26 NTD would improve understanding of this complex regulation. As a first step towards structural characterization, sequence specific (1)H, (13)C and (15)N assignments for MED26 NTD was performed based on Nuclear Magnetic Resonance spectroscopy. TALOS+ analysis of the chemical shifts data revealed a domain solely composed of helices. Assignments will be further used to solve NMR structure and dynamics of MED26 NTD and investigate the molecular details of its interaction with protein partners.
1H, 15N and 13C assignments of the N-terminal domain of the Mediator complex subunit MED26.,Peruzzini R, Lens Z, Verger A, Dewitte F, Ferreira E, Baert JL, Villeret V, Landrieu I, Cantrelle FX Biomol NMR Assign. 2016 Apr;10(1):233-6. doi: 10.1007/s12104-016-9673-z. Epub, 2016 Feb 9. PMID:26861138[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Peruzzini R, Lens Z, Verger A, Dewitte F, Ferreira E, Baert JL, Villeret V, Landrieu I, Cantrelle FX. 1H, 15N and 13C assignments of the N-terminal domain of the Mediator complex subunit MED26. Biomol NMR Assign. 2016 Apr;10(1):233-6. doi: 10.1007/s12104-016-9673-z. Epub, 2016 Feb 9. PMID:26861138 doi:http://dx.doi.org/10.1007/s12104-016-9673-z
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