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| | ==Structure of the Na,K-ATPase regulatory protein FXYD1 in micelles== | | ==Structure of the Na,K-ATPase regulatory protein FXYD1 in micelles== |
| - | <StructureSection load='2jo1' size='340' side='right' caption='[[2jo1]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2jo1' size='340' side='right'caption='[[2jo1]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2jo1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JO1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JO1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2jo1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JO1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JO1 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FXYD1, PLM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jo1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jo1 OCA], [http://pdbe.org/2jo1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jo1 RCSB], [http://www.ebi.ac.uk/pdbsum/2jo1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jo1 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jo1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jo1 OCA], [https://pdbe.org/2jo1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jo1 RCSB], [https://www.ebi.ac.uk/pdbsum/2jo1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jo1 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PLM_HUMAN PLM_HUMAN]] May have a functional role in muscle contraction. Induces a hyperpolarization-activated chloride current when exogenously expressed. | + | [https://www.uniprot.org/uniprot/PLM_HUMAN PLM_HUMAN] May have a functional role in muscle contraction. Induces a hyperpolarization-activated chloride current when exogenously expressed. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Choi, J]] | + | [[Category: Large Structures]] |
| - | [[Category: Franzin, C M]] | + | [[Category: Choi J]] |
| - | [[Category: Marassi, F M]] | + | [[Category: Franzin CM]] |
| - | [[Category: Teriete, P]] | + | [[Category: Marassi FM]] |
| - | [[Category: Fxyd1]] | + | [[Category: Teriete P]] |
| - | [[Category: Hydrolase regulator]]
| + | |
| - | [[Category: K-atpase]]
| + | |
| - | [[Category: Micelle]]
| + | |
| - | [[Category: Na]]
| + | |
| Structural highlights
Function
PLM_HUMAN May have a functional role in muscle contraction. Induces a hyperpolarization-activated chloride current when exogenously expressed.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
FXYD1 is a major regulatory subunit of the Na,K-ATPase and the principal substrate of hormone-regulated phosphorylation by c-AMP dependent protein kinases A and C in heart and skeletal muscle sarcolemma. It is a member of an evolutionarily conserved family of membrane proteins that regulate the function of the enzyme complex in a tissue-specific and physiological-state-specific manner. Here, we present the three-dimensional structure of FXYD1 determined in micelles by NMR spectroscopy. Structure determination was made possible by measuring residual dipolar couplings in weakly oriented micelle samples of the protein. This allowed us to obtain the relative orientations of the helical segments and information about the protein dynamics. The structural analysis was further facilitated by the inclusion of distance restraints, obtained from paramagnetic spin label relaxation enhancements, and by refinement with a micelle depth restraint, derived from paramagnetic Mn line broadening effects. The structure of FXYD1 provides the foundation for understanding its intra-membrane association with the Na,K-ATPase alpha subunit and suggests a mechanism whereby the phosphorylation of conserved Ser residues, by protein kinases A and C, could induce a conformational change in the cytoplasmic domain of the protein to modulate its interaction with the alpha subunit.
Structure of the Na,K-ATPase regulatory protein FXYD1 in micelles.,Teriete P, Franzin CM, Choi J, Marassi FM Biochemistry. 2007 Jun 12;46(23):6774-83. Epub 2007 May 19. PMID:17511473[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Teriete P, Franzin CM, Choi J, Marassi FM. Structure of the Na,K-ATPase regulatory protein FXYD1 in micelles. Biochemistry. 2007 Jun 12;46(23):6774-83. Epub 2007 May 19. PMID:17511473 doi:10.1021/bi700391b
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