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| - | ==Crystal structure of S. cerevisiae YNR046w, a zinc finger protein from the eRF1 methyltransferase complex.== | + | ==Structure of S. cerevisiae Trm112 protein, a methyltransferase activator== |
| - | <StructureSection load='2j6a' size='340' side='right' caption='[[2j6a]], [[Resolution|resolution]] 1.70Å' scene=''> | + | <StructureSection load='2j6a' size='340' side='right'caption='[[2j6a]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2j6a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J6A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J6A FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2j6a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J6A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J6A FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j6a OCA], [http://pdbe.org/2j6a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2j6a RCSB], [http://www.ebi.ac.uk/pdbsum/2j6a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2j6a ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j6a OCA], [https://pdbe.org/2j6a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j6a RCSB], [https://www.ebi.ac.uk/pdbsum/2j6a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j6a ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TR112_YEAST TR112_YEAST]] Together with MTQ2, required for the methylation of eRF1 on 'Gln-182'. Together with TRM11, required for the formation of 2-methylguanosine at position 10 in tRNA. Probably has additional functions.<ref>PMID:15899842</ref> <ref>PMID:17008308</ref> | + | [https://www.uniprot.org/uniprot/TR112_YEAST TR112_YEAST] Together with MTQ2, required for the methylation of eRF1 on 'Gln-182'. Together with TRM11, required for the formation of 2-methylguanosine at position 10 in tRNA. Probably has additional functions.<ref>PMID:15899842</ref> <ref>PMID:17008308</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 18824]] | + | [[Category: Large Structures]] |
| - | [[Category: Buckingham, R H]] | + | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: Champ, S]] | + | [[Category: Buckingham RH]] |
| - | [[Category: Graille, M]] | + | [[Category: Champ S]] |
| - | [[Category: Heurgue-Hamard, V]] | + | [[Category: Graille M]] |
| - | [[Category: Scrima, N]] | + | [[Category: Heurgue-Hamard V]] |
| - | [[Category: Tilbeurgh, H Van]]
| + | [[Category: Scrima N]] |
| - | [[Category: Ulryck, N]] | + | [[Category: Ulryck N]] |
| - | [[Category: Erf1]] | + | [[Category: Van Tilbeurgh H]] |
| - | [[Category: Methyltransferase]]
| + | |
| - | [[Category: Nuclear protein]]
| + | |
| - | [[Category: Protein methylation]]
| + | |
| - | [[Category: Transferase]]
| + | |
| - | [[Category: Translation termination]]
| + | |
| Structural highlights
Function
TR112_YEAST Together with MTQ2, required for the methylation of eRF1 on 'Gln-182'. Together with TRM11, required for the formation of 2-methylguanosine at position 10 in tRNA. Probably has additional functions.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Protein release factor eRF1 in Saccharomyces cerevisiae, in complex with eRF3 and GTP, is methylated on a functionally crucial Gln residue by the S-adenosylmethionine-dependent methyltransferase Ydr140w. Here we show that eRF1 methylation, in addition to these previously characterized components, requires a 15-kDa zinc-binding protein, Ynr046w. Co-expression in Escherichia coli of Ynr046w and Ydr140w allows the latter to be recovered in soluble form rather than as inclusion bodies, and the two proteins co-purify on nickel-nitrilotriacetic acid chromatography when Ydr140w alone carries a His tag. The crystal structure of Ynr046w has been determined to 1.7 A resolution. It comprises a zinc-binding domain built from both the N- and C-terminal sequences and an inserted domain, absent from bacterial and archaeal orthologs of the protein, composed of three alpha-helices. The active methyltransferase is the heterodimer Ydr140w.Ynr046w, but when alone, both in solution and in crystals, Ynr046w appears to be a homodimer. The Ynr046w eRF1 methyltransferase subunit is shared by the tRNA methyltransferase Trm11p and probably by two other enzymes containing a Rossman fold.
The zinc finger protein Ynr046w is plurifunctional and a component of the eRF1 methyltransferase in yeast.,Heurgue-Hamard V, Graille M, Scrima N, Ulryck N, Champ S, van Tilbeurgh H, Buckingham RH J Biol Chem. 2006 Nov 24;281(47):36140-8. Epub 2006 Sep 28. PMID:17008308[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Purushothaman SK, Bujnicki JM, Grosjean H, Lapeyre B. Trm11p and Trm112p are both required for the formation of 2-methylguanosine at position 10 in yeast tRNA. Mol Cell Biol. 2005 Jun;25(11):4359-70. PMID:15899842 doi:http://dx.doi.org/25/11/4359
- ↑ Heurgue-Hamard V, Graille M, Scrima N, Ulryck N, Champ S, van Tilbeurgh H, Buckingham RH. The zinc finger protein Ynr046w is plurifunctional and a component of the eRF1 methyltransferase in yeast. J Biol Chem. 2006 Nov 24;281(47):36140-8. Epub 2006 Sep 28. PMID:17008308 doi:10.1074/jbc.M608571200
- ↑ Heurgue-Hamard V, Graille M, Scrima N, Ulryck N, Champ S, van Tilbeurgh H, Buckingham RH. The zinc finger protein Ynr046w is plurifunctional and a component of the eRF1 methyltransferase in yeast. J Biol Chem. 2006 Nov 24;281(47):36140-8. Epub 2006 Sep 28. PMID:17008308 doi:10.1074/jbc.M608571200
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