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| | ==Pseudoazurin M16F== | | ==Pseudoazurin M16F== |
| - | <StructureSection load='2jkw' size='340' side='right' caption='[[2jkw]], [[Resolution|resolution]] 1.60Å' scene=''> | + | <StructureSection load='2jkw' size='340' side='right'caption='[[2jkw]], [[Resolution|resolution]] 1.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2jkw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"achromobacter_cycloclastes"_(gray_and_thornton)_bergey_et_al. "achromobacter cycloclastes" (gray and thornton) bergey et al.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JKW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JKW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2jkw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Achromobacter_cycloclastes Achromobacter cycloclastes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JKW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JKW FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1zia|1zia]], [[1zib|1zib]], [[2ux7|2ux7]], [[2uxf|2uxf]], [[2ux6|2ux6]], [[1bqr|1bqr]], [[1bqk|1bqk]], [[2uxg|2uxg]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jkw OCA], [http://pdbe.org/2jkw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jkw RCSB], [http://www.ebi.ac.uk/pdbsum/2jkw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jkw ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jkw OCA], [https://pdbe.org/2jkw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jkw RCSB], [https://www.ebi.ac.uk/pdbsum/2jkw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jkw ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/AZUP_ACHCY AZUP_ACHCY]] This soluble electron transfer copper protein is required for the inactivation of copper-containing nitrite reductase in the presence of oxygen. | + | [https://www.uniprot.org/uniprot/AZUP_ACHCY AZUP_ACHCY] This soluble electron transfer copper protein is required for the inactivation of copper-containing nitrite reductase in the presence of oxygen. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Banfield, M J]] | + | [[Category: Achromobacter cycloclastes]] |
| - | [[Category: Crowley, P B]] | + | [[Category: Large Structures]] |
| - | [[Category: Dennison, C]] | + | [[Category: Banfield MJ]] |
| - | [[Category: Firbank, S J]] | + | [[Category: Crowley PB]] |
| - | [[Category: Hunter, D M]] | + | [[Category: Dennison C]] |
| - | [[Category: Kohzuma, T]] | + | [[Category: Firbank SJ]] |
| - | [[Category: Lawler, A T]] | + | [[Category: Hunter DM]] |
| - | [[Category: Li, C]] | + | [[Category: Kohzuma T]] |
| - | [[Category: McFarlane, W]] | + | [[Category: Lawler AT]] |
| - | [[Category: Yanagisawa, S]] | + | [[Category: Li C]] |
| - | [[Category: Copper]]
| + | [[Category: McFarlane W]] |
| - | [[Category: Cupredoxin]]
| + | [[Category: Yanagisawa S]] |
| - | [[Category: Electron transfer]]
| + | |
| - | [[Category: Electron transport]]
| + | |
| - | [[Category: Metal-binding]]
| + | |
| - | [[Category: Periplasm]]
| + | |
| - | [[Category: Pi- interaction]]
| + | |
| - | [[Category: Pseudoazurin]]
| + | |
| - | [[Category: Transport]]
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| Structural highlights
Function
AZUP_ACHCY This soluble electron transfer copper protein is required for the inactivation of copper-containing nitrite reductase in the presence of oxygen.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The influence of pi-interactions with a His ligand have been investigated in a family of copper-containing redox metalloproteins. The Met16Phe and Met16Trp pseudoazurin, and Leu12Phe spinach and Leu14Phe Phormidium laminosum plastocyanin variants possess active-site pi-contacts between the introduced residue and His81 and His87/92 respectively. The striking overlap of the side chain of Phe16 in the Met16Phe variant and that of Met16 in wild type pseudoazurin identifies that this position provides an important second coordination sphere interaction in both cases. His-ligand protonation and dissociation from Cu(I) occurs in the wild type proteins resulting in diminished redox activity, providing a [H (+)]-driven switch for regulating electron transfer. The introduced pi-interaction has opposing effects on the p K a for the His ligand in pseudoazurin and plastocyanin due to subtle differences in the pi-contact, stabilizing the coordinated form of pseudoazurin whereas in plastocyanin protonation and dissociation is favored. Replacement of Pro36, a residue that has been suggested to facilitate structural changes upon His ligand protonation, with a Gly, has little effect on the p K a of His87 in spinach plastocyanin. The mutations at Met16 have a significant influence on the reduction potential of pseudoazurin. Electron self-exchange is enhanced, whereas association with the physiological partner, nitrite reductase, is only affected by the Met16Phe mutation, but k cat is halved in both the Met16Phe and Met16Trp variants. Protonation of the His ligand is the feature most affected by the introduction of a pi-interaction.
pi-Interaction Tuning of the Active Site Properties of Metalloproteins.,Yanagisawa S, Crowley PB, Firbank SJ, Lawler AT, Hunter DM, McFarlane W, Li C, Kohzuma T, Banfield MJ, Dennison C J Am Chem Soc. 2008 Oct 22. PMID:18939838[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Yanagisawa S, Crowley PB, Firbank SJ, Lawler AT, Hunter DM, McFarlane W, Li C, Kohzuma T, Banfield MJ, Dennison C. pi-Interaction Tuning of the Active Site Properties of Metalloproteins. J Am Chem Soc. 2008 Oct 22. PMID:18939838 doi:10.1021/ja8038135
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