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| | ==CORE BINDING FACTOR BETA== | | ==CORE BINDING FACTOR BETA== |
| - | <StructureSection load='2jhb' size='340' side='right' caption='[[2jhb]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2jhb' size='340' side='right'caption='[[2jhb]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2jhb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JHB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JHB FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2jhb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JHB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JHB FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CBFB141 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jhb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jhb OCA], [http://pdbe.org/2jhb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jhb RCSB], [http://www.ebi.ac.uk/pdbsum/2jhb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jhb ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jhb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jhb OCA], [https://pdbe.org/2jhb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jhb RCSB], [https://www.ebi.ac.uk/pdbsum/2jhb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jhb ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PEBB_MOUSE PEBB_MOUSE]] CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBFB enhances DNA binding by RUNX1. | + | [https://www.uniprot.org/uniprot/PEBB_MOUSE PEBB_MOUSE] CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBFB enhances DNA binding by RUNX1. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </div> | | </div> |
| | <div class="pdbe-citations 2jhb" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 2jhb" style="background-color:#fffaf0;"></div> |
| - | | |
| - | ==See Also== | |
| - | *[[Core-binding factor|Core-binding factor]] | |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Bushweller, J H]] | + | [[Category: Mus musculus]] |
| - | [[Category: Huang, X]] | + | [[Category: Bushweller JH]] |
| - | [[Category: Peng, J]] | + | [[Category: Huang X]] |
| - | [[Category: Speck, N A]] | + | [[Category: Peng J]] |
| - | [[Category: Core binding factor]] | + | [[Category: Speck NA]] |
| - | [[Category: Gene regulation]]
| + | |
| - | [[Category: Transcription factor]]
| + | |
| Structural highlights
Function
PEBB_MOUSE CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBFB enhances DNA binding by RUNX1.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The core binding factor beta subunit (CBF beta) is the non-DNA binding subunit of the core-binding factors, transcription factors essential for multiple developmental processes including hematopoiesis and bone development. Chromosomal translocations involving the human CBFB gene are associated with a large percentage of human leukemias. The N-terminal 141 amino acids of CBF beta contains the heterodimerization domain for the DNA-binding CBF alpha subunits, and is sufficient for CBF beta function in vivo. Here we present the high-resolution solution structure of the CBF beta heterodimerization domain. It is a novel alpha/beta structure consisting of two three-stranded beta-sheets packed on one another in a sandwich arrangement, with four peripheral alpha-helices. The CBF alpha binding site on CBF beta has been mapped by chemical shift perturbation analysis.
Solution structure of core binding factor beta and map of the CBF alpha binding site.,Huang X, Peng JW, Speck NA, Bushweller JH Nat Struct Biol. 1999 Jul;6(7):624-7. PMID:10404216[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Huang X, Peng JW, Speck NA, Bushweller JH. Solution structure of core binding factor beta and map of the CBF alpha binding site. Nat Struct Biol. 1999 Jul;6(7):624-7. PMID:10404216 doi:10.1038/10670
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