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| | ==Solution structure of fly troponin C, isoform F1== | | ==Solution structure of fly troponin C, isoform F1== |
| - | <StructureSection load='2jnf' size='340' side='right' caption='[[2jnf]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='2jnf' size='340' side='right'caption='[[2jnf]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2jnf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lethocerus_indica Lethocerus indica]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JNF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JNF FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2jnf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lethocerus_indicus Lethocerus indicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JNF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JNF FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tnC4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=212017 Lethocerus indica])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jnf OCA], [http://pdbe.org/2jnf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jnf RCSB], [http://www.ebi.ac.uk/pdbsum/2jnf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jnf ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jnf OCA], [https://pdbe.org/2jnf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jnf RCSB], [https://www.ebi.ac.uk/pdbsum/2jnf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jnf ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q868D4_9HEMI Q868D4_9HEMI] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | ==See Also== | | ==See Also== |
| - | *[[Troponin|Troponin]] | + | *[[Troponin 3D structures|Troponin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lethocerus indica]] | + | [[Category: Large Structures]] |
| - | [[Category: Bullard, B]]
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| - | [[Category: Nicola, G F.De]]
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| - | [[Category: Pastore, A]]
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| - | [[Category: Ef-hand]]
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| | [[Category: Lethocerus indicus]] | | [[Category: Lethocerus indicus]] |
| - | [[Category: Metal binding protein]] | + | [[Category: Bullard B]] |
| - | [[Category: Stretch activated muscle contraction]] | + | [[Category: De Nicola GF]] |
| - | [[Category: Troponin c]] | + | [[Category: Pastore A]] |
| Structural highlights
Function
Q868D4_9HEMI
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
To gain a molecular description of how muscles can be activated by mechanical stretch, we have solved the structure of the calcium-loaded F1 isoform of troponin C (TnC) from Lethocerus and characterized its interactions with troponin I (TnI). We show that the presence of only one calcium cation in the fourth EF hand motif is sufficient to induce an open conformation in the C-terminal lobe of F1 TnC, in contrast with what is observed in vertebrate muscle. This lobe interacts in a calcium-independent way both with the N terminus of TnI and, with lower affinity, with a region of TnI equivalent to the switch and inhibitory peptides of vertebrate muscles. Using both synthetic peptides and recombinant proteins, we show that the N lobe of F1 TnC is not engaged in interactions with TnI, excluding a regulatory role of this domain. These findings provide insights into mechanically stimulated muscle contraction.
The structure of Lethocerus troponin C: insights into the mechanism of stretch activation in muscles.,De Nicola G, Burkart C, Qiu F, Agianian B, Labeit S, Martin S, Bullard B, Pastore A Structure. 2007 Jul;15(7):813-24. PMID:17637342[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ De Nicola G, Burkart C, Qiu F, Agianian B, Labeit S, Martin S, Bullard B, Pastore A. The structure of Lethocerus troponin C: insights into the mechanism of stretch activation in muscles. Structure. 2007 Jul;15(7):813-24. PMID:17637342 doi:10.1016/j.str.2007.05.007
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