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| | ==Solution Structure of GB1 domain Protein G and low and high pressure.== | | ==Solution Structure of GB1 domain Protein G and low and high pressure.== |
| - | <StructureSection load='2j53' size='340' side='right' caption='[[2j53]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''> | + | <StructureSection load='2j53' size='340' side='right'caption='[[2j53]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2j53]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strsp Strsp]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J53 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J53 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2j53]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_sp. Streptococcus sp.]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J53 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J53 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1em7|1em7]], [[1gb1|1gb1]], [[1igc|1igc]], [[1igd|1igd]], [[1le3|1le3]], [[1mpe|1mpe]], [[1mvk|1mvk]], [[1pga|1pga]], [[1pgb|1pgb]], [[1pgx|1pgx]], [[1pn5|1pn5]], [[1q10|1q10]], [[2gb1|2gb1]], [[2igd|2igd]], [[2igh|2igh]], [[2j52|2j52]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j53 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j53 OCA], [http://pdbe.org/2j53 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2j53 RCSB], [http://www.ebi.ac.uk/pdbsum/2j53 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2j53 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j53 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j53 OCA], [https://pdbe.org/2j53 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j53 RCSB], [https://www.ebi.ac.uk/pdbsum/2j53 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j53 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/SPG1_STRSG SPG1_STRSG] Binds to the constant Fc region of IgG with high affinity. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </div> | | </div> |
| | <div class="pdbe-citations 2j53" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 2j53" style="background-color:#fffaf0;"></div> |
| - | | |
| - | ==See Also== | |
| - | *[[Protein G|Protein G]] | |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Strsp]] | + | [[Category: Large Structures]] |
| - | [[Category: Akasaka, K]] | + | [[Category: Streptococcus sp]] |
| - | [[Category: Kamatari, Y O]] | + | [[Category: Akasaka K]] |
| - | [[Category: Tunnicliffe, R B]] | + | [[Category: Kamatari YO]] |
| - | [[Category: Williamson, M P]] | + | [[Category: Tunnicliffe RB]] |
| - | [[Category: Wilton, D J]] | + | [[Category: Williamson MP]] |
| - | [[Category: Cell wall]] | + | [[Category: Wilton DJ]] |
| - | [[Category: Igg-binding protein]]
| + | |
| - | [[Category: Immunoglobulin]]
| + | |
| - | [[Category: Peptidoglycan-anchor]]
| + | |
| - | [[Category: Pressure]]
| + | |
| - | [[Category: Protein g]]
| + | |
| Structural highlights
Function
SPG1_STRSG Binds to the constant Fc region of IgG with high affinity.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The solution structure of the GB1 domain of protein G at a pressure of 2 kbar is presented. The structure was calculated as a change from an energy-minimised low-pressure structure using (1)H chemical shifts. Two separate changes can be characterised: a compression/distortion, which is linear with pressure; and a stabilisation of an alternative folded state. On application of pressure, linear chemical shift changes reveal that the backbone structure changes by about 0.2 A root mean square, and is compressed by about 1% overall. The alpha-helix compresses, particularly at the C-terminal end, and moves toward the beta-sheet, while the beta-sheet is twisted, with the corners closest to the alpha-helix curling up towards it. The largest changes in structure are along the second beta-strand, which becomes more twisted. This strand is where the protein binds to IgG. Curved chemical shift changes with pressure indicate that high pressure also populates an alternative structure with a distortion towards the C-terminal end of the helix, which is likely to be caused by insertion of a water molecule. Proteins 2007. (c) 2007 Wiley-Liss, Inc.
Pressure-induced changes in the solution structure of the GB1 domain of protein G.,Wilton DJ, Tunnicliffe RB, Kamatari YO, Akasaka K, Williamson MP Proteins. 2007 Dec 12;. PMID:18076052[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wilton DJ, Tunnicliffe RB, Kamatari YO, Akasaka K, Williamson MP. Pressure-induced changes in the solution structure of the GB1 domain of protein G. Proteins. 2007 Dec 12;. PMID:18076052 doi:10.1002/prot.21832
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