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| | ==Structure of the yeast Pml1 splicing factor and its integration into the RES complex== | | ==Structure of the yeast Pml1 splicing factor and its integration into the RES complex== |
| - | <StructureSection load='2jkd' size='340' side='right' caption='[[2jkd]], [[Resolution|resolution]] 2.50Å' scene=''> | + | <StructureSection load='2jkd' size='340' side='right'caption='[[2jkd]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2jkd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JKD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JKD FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2jkd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JKD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JKD FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jkd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jkd OCA], [http://pdbe.org/2jkd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jkd RCSB], [http://www.ebi.ac.uk/pdbsum/2jkd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jkd ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jkd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jkd OCA], [https://pdbe.org/2jkd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jkd RCSB], [https://www.ebi.ac.uk/pdbsum/2jkd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jkd ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/PML1_YEAST PML1_YEAST] Required for efficient splicing and pre-mRNA nuclear retention.<ref>PMID:15565172</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 18824]] | + | [[Category: Large Structures]] |
| - | [[Category: Brooks, M A]]
| + | |
| - | [[Category: Dziembowski, A]]
| + | |
| - | [[Category: Faux, C]]
| + | |
| - | [[Category: Henriot, V]]
| + | |
| - | [[Category: Quevillon-Cheruel, S]]
| + | |
| - | [[Category: Seraphin, B]]
| + | |
| - | [[Category: Tilbeurgh, H van]]
| + | |
| - | [[Category: Gene regulation]]
| + | |
| - | [[Category: Mrna processing]]
| + | |
| - | [[Category: Mrna splicing]]
| + | |
| - | [[Category: Nucleus]]
| + | |
| - | [[Category: Phospho-peptide recognition]]
| + | |
| - | [[Category: Pml1/snu17/bud13]]
| + | |
| - | [[Category: Pre-mrna splicing]]
| + | |
| - | [[Category: Re]]
| + | |
| | [[Category: Saccharomyces cerevisiae]] | | [[Category: Saccharomyces cerevisiae]] |
| | + | [[Category: Brooks MA]] |
| | + | [[Category: Dziembowski A]] |
| | + | [[Category: Faux C]] |
| | + | [[Category: Henriot V]] |
| | + | [[Category: Quevillon-Cheruel S]] |
| | + | [[Category: Seraphin B]] |
| | + | [[Category: Van Tilbeurgh H]] |
| Structural highlights
Function
PML1_YEAST Required for efficient splicing and pre-mRNA nuclear retention.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The RES complex was previously identified in yeast as a splicing factor affecting nuclear pre-mRNA retention. This complex was shown to contain three subunits, namely Snu17, Bud13 and Pml1, but its mode of action remains ill-defined. To obtain insights into its function, we have performed a structural investigation of this factor. Production of a short N-terminal truncation of residues that are apparently disordered allowed us to determine the X-ray crystallographic structure of Pml1. This demonstrated that it consists mainly of a FHA domain, a fold which has been shown to mediate interactions with phosphothreonine-containing peptides. Using a new sensitive assay based on alternative splice-site choice, we show, however, that mutation of the putative phosphothreonine-binding pocket of Pml1 does not affect pre-mRNA splicing. We have also investigated how Pml1 integrates into the RES complex. Production of recombinant complexes, combined with serial truncation and mutagenesis of their subunits, indicated that Pml1 binds to Snu17, which itself contacts Bud13. This analysis allowed us to demarcate the binding sites involved in the formation of this assembly. We propose a model of the organization of the RES complex based on these results, and discuss the functional consequences of this architecture.
Structure of the yeast Pml1 splicing factor and its integration into the RES complex.,Brooks MA, Dziembowski A, Quevillon-Cheruel S, Henriot V, Faux C, van Tilbeurgh H, Seraphin B Nucleic Acids Res. 2009 Jan;37(1):129-43. Epub 2008 Nov 25. PMID:19033360[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Dziembowski A, Ventura AP, Rutz B, Caspary F, Faux C, Halgand F, Laprevote O, Seraphin B. Proteomic analysis identifies a new complex required for nuclear pre-mRNA retention and splicing. EMBO J. 2004 Dec 8;23(24):4847-56. Epub 2004 Nov 25. PMID:15565172 doi:http://dx.doi.org/7600482
- ↑ Brooks MA, Dziembowski A, Quevillon-Cheruel S, Henriot V, Faux C, van Tilbeurgh H, Seraphin B. Structure of the yeast Pml1 splicing factor and its integration into the RES complex. Nucleic Acids Res. 2009 Jan;37(1):129-43. Epub 2008 Nov 25. PMID:19033360 doi:10.1093/nar/gkn894
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