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| - | {{Large structure}}
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| | ==Architectural model of the type IVa pilus machine in a non-piliated state== | | ==Architectural model of the type IVa pilus machine in a non-piliated state== |
| - | <StructureSection load='3jc9' size='340' side='right' caption='[[3jc9]]' scene=''> | + | <SX load='3jc9' size='340' side='right' viewer='molstar' caption='[[3jc9]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3jc9]] is a 79 chain structure with sequence from [http://en.wikipedia.org/wiki/Myxococcus_xanthus_dk_1622 Myxococcus xanthus dk 1622]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JC9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JC9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3jc9]] is a 79 chain structure with sequence from [https://en.wikipedia.org/wiki/Myxococcus_xanthus_DK_1622 Myxococcus xanthus DK 1622]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JC9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JC9 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MEA:N-METHYLPHENYLALANINE'>MEA</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MEA:N-METHYLPHENYLALANINE'>MEA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3jc8|3jc8]]</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jc9 OCA], [https://pdbe.org/3jc9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jc9 RCSB], [https://www.ebi.ac.uk/pdbsum/3jc9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jc9 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3jc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jc9 OCA], [http://pdbe.org/3jc9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3jc9 RCSB], [http://www.ebi.ac.uk/pdbsum/3jc9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3jc9 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | {{Large structure}}
| + | == Function == |
| | + | [https://www.uniprot.org/uniprot/PILC_MYXXD PILC_MYXXD] Essential inner membrane component of the type IV pilus (T4P) that plays a role in surface and host cell adhesion, colonization, biofilm maturation, virulence, and twitching, a form of surface-associated motility facilitated by cycles of extension, adhesion, and retraction of T4P fibers (By similarity). Controls both pilus assembly and disassembly and plays an important role in PilB localization to the complex and ATPase activity (PubMed:26851283).[UniProtKB:P22609]<ref>PMID:26851283</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| - | </StructureSection> | + | </SX> |
| - | [[Category: Myxococcus xanthus dk 1622]] | + | [[Category: Large Structures]] |
| - | [[Category: Chang, Y W]] | + | [[Category: Myxococcus xanthus DK 1622]] |
| - | [[Category: Jensen, G J]] | + | [[Category: Chang Y-W]] |
| - | [[Category: Rettberg, L A]] | + | [[Category: Jensen GJ]] |
| - | [[Category: Membrane channel]]
| + | [[Category: Rettberg LA]] |
| - | [[Category: Motor]]
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| - | [[Category: Motor protein]]
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| - | [[Category: Pilus]]
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| - | [[Category: Ring]]
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| Structural highlights
Function
PILC_MYXXD Essential inner membrane component of the type IV pilus (T4P) that plays a role in surface and host cell adhesion, colonization, biofilm maturation, virulence, and twitching, a form of surface-associated motility facilitated by cycles of extension, adhesion, and retraction of T4P fibers (By similarity). Controls both pilus assembly and disassembly and plays an important role in PilB localization to the complex and ATPase activity (PubMed:26851283).[UniProtKB:P22609][1]
Publication Abstract from PubMed
Type IVa pili are filamentous cell surface structures observed in many bacteria. They pull cells forward by extending, adhering to surfaces, and then retracting. We used cryo-electron tomography of intact Myxococcus xanthus cells to visualize type IVa pili and the protein machine that assembles and retracts them (the type IVa pilus machine, or T4PM) in situ, in both the piliated and nonpiliated states, at a resolution of 3 to 4 nanometers. We found that T4PM comprises an outer membrane pore, four interconnected ring structures in the periplasm and cytoplasm, a cytoplasmic disc and dome, and a periplasmic stem. By systematically imaging mutants lacking defined T4PM proteins or with individual proteins fused to tags, we mapped the locations of all 10 T4PM core components and the minor pilins, thereby providing insights into pilus assembly, structure, and function.
Architecture of the type IVa pilus machine.,Chang YW, Rettberg LA, Treuner-Lange A, Iwasa J, Sogaard-Andersen L, Jensen GJ Science. 2016 Mar 11;351(6278):aad2001. doi: 10.1126/science.aad2001. Epub 2016, Mar 10. PMID:26965631[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bischof LF, Friedrich C, Harms A, Søgaard-Andersen L, van der Does C. The Type IV Pilus Assembly ATPase PilB of Myxococcus xanthus Interacts with the Inner Membrane Platform Protein PilC and the Nucleotide-binding Protein PilM. J Biol Chem. 2016 Mar 25;291(13):6946-57. PMID:26851283 doi:10.1074/jbc.M115.701284
- ↑ Chang YW, Rettberg LA, Treuner-Lange A, Iwasa J, Sogaard-Andersen L, Jensen GJ. Architecture of the type IVa pilus machine. Science. 2016 Mar 11;351(6278):aad2001. doi: 10.1126/science.aad2001. Epub 2016, Mar 10. PMID:26965631 doi:http://dx.doi.org/10.1126/science.aad2001
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