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| | ==Structure of the STRA6 receptor for retinol uptake in complex with calmodulin== | | ==Structure of the STRA6 receptor for retinol uptake in complex with calmodulin== |
| - | <StructureSection load='5sy1' size='340' side='right' caption='[[5sy1]], [[Resolution|resolution]] 3.90Å' scene=''> | + | <SX load='5sy1' size='340' side='right' viewer='molstar' caption='[[5sy1]], [[Resolution|resolution]] 3.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5sy1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Brachidanio_rerio Brachidanio rerio] and [http://en.wikipedia.org/wiki/Spodoptera_frugiperda Spodoptera frugiperda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5SY1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5SY1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5sy1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Danio_rerio Danio rerio] and [https://en.wikipedia.org/wiki/Spodoptera_frugiperda Spodoptera frugiperda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5SY1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5SY1 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.9Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">stra6, zgc:136689 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7955 Brachidanio rerio])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5sy1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5sy1 OCA], [http://pdbe.org/5sy1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5sy1 RCSB], [http://www.ebi.ac.uk/pdbsum/5sy1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5sy1 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5sy1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5sy1 OCA], [https://pdbe.org/5sy1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5sy1 RCSB], [https://www.ebi.ac.uk/pdbsum/5sy1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5sy1 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/A0A1C7D1B9_SPOFR A0A1C7D1B9_SPOFR] |
| - | Vitamin A homeostasis is critical to normal cellular function. Retinol-binding protein (RBP) is the sole specific carrier in the bloodstream for hydrophobic retinol, the main form in which vitamin A is transported. The integral membrane receptor STRA6 mediates cellular uptake of vitamin A by recognizing RBP-retinol to trigger release and internalization of retinol. We present the structure of zebrafish STRA6 determined to 3.9-angstrom resolution by single-particle cryo-electron microscopy. STRA6 has one intramembrane and nine transmembrane helices in an intricate dimeric assembly. Unexpectedly, calmodulin is bound tightly to STRA6 in a noncanonical arrangement. Residues involved with RBP binding map to an archlike structure that covers a deep lipophilic cleft. This cleft is open to the membrane, suggesting a possible mode for internalization of retinol through direct diffusion into the lipid bilayer.
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| - | Structure of the STRA6 receptor for retinol uptake.,Chen Y, Clarke OB, Kim J, Stowe S, Kim YK, Assur Z, Cavalier M, Godoy-Ruiz R, von Alpen DC, Manzini C, Blaner WS, Frank J, Quadro L, Weber DJ, Shapiro L, Hendrickson WA, Mancia F Science. 2016 Aug 26;353(6302). pii: aad8266. doi: 10.1126/science.aad8266. PMID:27563101<ref>PMID:27563101</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 5sy1" style="background-color:#fffaf0;"></div>
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| | ==See Also== | | ==See Also== |
| - | *[[Calmodulin|Calmodulin]] | + | *[[Calmodulin 3D structures|Calmodulin 3D structures]] |
| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| - | </StructureSection> | + | </SX> |
| - | [[Category: Brachidanio rerio]] | + | [[Category: Danio rerio]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Spodoptera frugiperda]] | | [[Category: Spodoptera frugiperda]] |
| - | [[Category: Chen, Y]] | + | [[Category: Chen Y]] |
| - | [[Category: Clarke, O B]] | + | [[Category: Clarke OB]] |
| - | [[Category: Mancia, F]] | + | [[Category: Mancia F]] |
| - | [[Category: Membrane]]
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| - | [[Category: Membrane protein-calcium binding protein complex]]
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| - | [[Category: Retinol]]
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| - | [[Category: Stra6]]
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| - | [[Category: Vitamin some]]
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