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| | ==Structure of the fifth zinc finger of Myelin Transcription Factor 1== | | ==Structure of the fifth zinc finger of Myelin Transcription Factor 1== |
| - | <StructureSection load='2jyd' size='340' side='right' caption='[[2jyd]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2jyd' size='340' side='right'caption='[[2jyd]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2jyd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JYD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JYD FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2jyd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JYD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JYD FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jyd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jyd OCA], [http://pdbe.org/2jyd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jyd RCSB], [http://www.ebi.ac.uk/pdbsum/2jyd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jyd ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jyd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jyd OCA], [https://pdbe.org/2jyd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jyd RCSB], [https://www.ebi.ac.uk/pdbsum/2jyd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jyd ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MYT1_MOUSE MYT1_MOUSE]] Binds to the promoter regions of proteolipid proteins of the central nervous system. May play a role in the development of neurons and oligodendrogalia in the CNS. May regulate a critical transition point in oligodendrocyte lineage development by modulating oligodendrocyte progenitor proliferation relative to terminal differentiation and up-regulation of myelin gene transcription (By similarity).<ref>PMID:9373037</ref> | + | [https://www.uniprot.org/uniprot/MYT1_MOUSE MYT1_MOUSE] Binds to the promoter regions of proteolipid proteins of the central nervous system. May play a role in the development of neurons and oligodendrogalia in the CNS. May regulate a critical transition point in oligodendrocyte lineage development by modulating oligodendrocyte progenitor proliferation relative to terminal differentiation and up-regulation of myelin gene transcription (By similarity).<ref>PMID:9373037</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Gamsjaeger, R]] | + | [[Category: Mus musculus]] |
| - | [[Category: Kobus, F J]] | + | [[Category: Gamsjaeger R]] |
| - | [[Category: Kwan, A H]] | + | [[Category: Kobus FJ]] |
| - | [[Category: Lehtomaki, E]] | + | [[Category: Kwan AH]] |
| - | [[Category: Lowry, J A]] | + | [[Category: Lehtomaki E]] |
| - | [[Category: Mackay, J P]] | + | [[Category: Lowry JA]] |
| - | [[Category: Matthews, J M]] | + | [[Category: Mackay JP]] |
| - | [[Category: Swanton, M K]] | + | [[Category: Matthews JM]] |
| - | [[Category: Alternative splicing]]
| + | [[Category: Swanton MK]] |
| - | [[Category: Developmental protein]]
| + | |
| - | [[Category: Differentiation]]
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| - | [[Category: Dna-binding]]
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| - | [[Category: Metal binding protein]]
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| - | [[Category: Metal-binding]]
| + | |
| - | [[Category: Myt1]]
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| - | [[Category: Neurogenesis]]
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| - | [[Category: Nucleus]]
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| - | [[Category: Transcription]]
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| - | [[Category: Transcription regulation]]
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| - | [[Category: Zinc-finger]]
| + | |
| Structural highlights
Function
MYT1_MOUSE Binds to the promoter regions of proteolipid proteins of the central nervous system. May play a role in the development of neurons and oligodendrogalia in the CNS. May regulate a critical transition point in oligodendrocyte lineage development by modulating oligodendrocyte progenitor proliferation relative to terminal differentiation and up-regulation of myelin gene transcription (By similarity).[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Zinc binding domains, or zinc fingers (ZnFs), form one of the most numerous and most diverse superclasses of protein structural motifs in eukaryotes. Although our understanding of the functions of several classes of these domains is relatively well developed, we know much less about the molecular mechanisms of action of many others. Myelin transcription factor 1 (MyT1) type ZnFs are found in organisms as diverse as nematodes and mammals and are found in a range of sequence contexts. MyT1, one of the early transcription factors expressed in the developing central nervous system, contains seven MyT1 ZnFs that are very highly conserved both within the protein and between species. We have used a range of biophysical techniques, including NMR spectroscopy and data-driven macromolecular docking, to investigate the structural basis for the interaction between MyT1 ZnFs and DNA. Our data indicate that MyT1 ZnFs recognize the major groove of DNA in a way that appears to differ from other known zinc binding domains.
Structural and biophysical analysis of the DNA binding properties of myelin transcription factor 1.,Gamsjaeger R, Swanton MK, Kobus FJ, Lehtomaki E, Lowry JA, Kwan AH, Matthews JM, Mackay JP J Biol Chem. 2008 Feb 22;283(8):5158-67. Epub 2007 Dec 11. PMID:18073212[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kim JG, Armstrong RC, v Agoston D, Robinsky A, Wiese C, Nagle J, Hudson LD. Myelin transcription factor 1 (Myt1) of the oligodendrocyte lineage, along with a closely related CCHC zinc finger, is expressed in developing neurons in the mammalian central nervous system. J Neurosci Res. 1997 Oct 15;50(2):272-90. PMID:9373037
- ↑ Gamsjaeger R, Swanton MK, Kobus FJ, Lehtomaki E, Lowry JA, Kwan AH, Matthews JM, Mackay JP. Structural and biophysical analysis of the DNA binding properties of myelin transcription factor 1. J Biol Chem. 2008 Feb 22;283(8):5158-67. Epub 2007 Dec 11. PMID:18073212 doi:10.1074/jbc.M703772200
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