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| | ==NMR structure of human Serine protease inhibitor Kazal type II (SPINK2)== | | ==NMR structure of human Serine protease inhibitor Kazal type II (SPINK2)== |
| - | <StructureSection load='2jxd' size='340' side='right' caption='[[2jxd]], [[NMR_Ensembles_of_Models | 15 NMR models]]' scene=''> | + | <StructureSection load='2jxd' size='340' side='right'caption='[[2jxd]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2jxd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JXD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JXD FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2jxd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JXD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JXD FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SPINK2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 15 models</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jxd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jxd OCA], [http://pdbe.org/2jxd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jxd RCSB], [http://www.ebi.ac.uk/pdbsum/2jxd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jxd ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jxd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jxd OCA], [https://pdbe.org/2jxd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jxd RCSB], [https://www.ebi.ac.uk/pdbsum/2jxd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jxd ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ISK2_HUMAN ISK2_HUMAN]] Strong inhibitor of acrosin in male and/or female genital tract. Also inhibits trypsin.<ref>PMID:19422058</ref> | + | [https://www.uniprot.org/uniprot/ISK2_HUMAN ISK2_HUMAN] Strong inhibitor of acrosin in male and/or female genital tract. Also inhibits trypsin.<ref>PMID:19422058</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | <jmolCheckbox> | | <jmolCheckbox> |
| | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jx/2jxd_consurf.spt"</scriptWhenChecked> | | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jx/2jxd_consurf.spt"</scriptWhenChecked> |
| - | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> | | </jmolCheckbox> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Chen, T]] | + | [[Category: Large Structures]] |
| - | [[Category: Lee, T R]] | + | [[Category: Chen T]] |
| - | [[Category: Lyu, P C]] | + | [[Category: Lee T-R]] |
| - | [[Category: Alpha helix]]
| + | [[Category: Lyu P-C]] |
| - | [[Category: Anti-parallel beta sheet]]
| + | |
| - | [[Category: Beta-bulge]] | + | |
| - | [[Category: Disulfide bond]]
| + | |
| - | [[Category: Hydrolase inhibitor]]
| + | |
| - | [[Category: Protease inhibitor]]
| + | |
| - | [[Category: Pyrrolidone carboxylic acid]]
| + | |
| - | [[Category: Secreted]]
| + | |
| - | [[Category: Serine protease inhibitor]]
| + | |
| Structural highlights
Function
ISK2_HUMAN Strong inhibitor of acrosin in male and/or female genital tract. Also inhibits trypsin.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Human serine proteinase inhibitor Kazal-type 2 (SPINK2) functions as a trypsin/acrosin inhibitor and is synthesized mainly in the testis and seminal vesicle where its activity is engaged in fertility. The SPINK2 protein contains a typical Kazal domain composed by six cysteine residues forming three disulfide bridges. The expression of SPINK2 is closely related to cancer such as lymphomas, in that a high transcript level of SPINK2 in patients with primary cutaneous follicle center cell lymphomas have better prognosis with lower mortality. To clarify the role of SPINK2 in cancer, we performed quantitative real-time PCR and showed that the expression level of SPINK2 is significantly elevated in most leukemia cell lines except B-lymphoblast TK-6 cells. The molecular function and structural features of SPINK2 were also investigated by employing the recombinant active and mutant inactive SPINK2 proteins to determine its key P2-P2' (Pro(23)-Arg(24)-His(25)-Phe(26)) active site. The inhibition assay results demonstrated that Arg(24) at the P1 site is crucial for the specificity of SPINK2 on target enzyme. Although His(25) at the P1' and Phe(26) at the P2' residues are also involved in trypsin-SPINK2 interaction, Pro(23) at the P2 site may not be directly participated in interacting with trypsin. In addition, we determined the 3D solution structure of SPINK2 and used this structure to predict the SPINK2-proteinase complex structure and binding properties. These studies not only provide critical information about the structural properties and biophysical features of the SPINK2 proteinase inhibitor, but also suggest its important role in tumor progression and response to treatment.
Identification of trypsin-inhibitory site and structure determination of human SPINK2 serine proteinase inhibitor.,Chen T, Lee TR, Liang WG, Chang WS, Lyu PC Proteins. 2009 Oct;77(1):209-19. PMID:19422058[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Chen T, Lee TR, Liang WG, Chang WS, Lyu PC. Identification of trypsin-inhibitory site and structure determination of human SPINK2 serine proteinase inhibitor. Proteins. 2009 Oct;77(1):209-19. PMID:19422058 doi:10.1002/prot.22432
- ↑ Chen T, Lee TR, Liang WG, Chang WS, Lyu PC. Identification of trypsin-inhibitory site and structure determination of human SPINK2 serine proteinase inhibitor. Proteins. 2009 Oct;77(1):209-19. PMID:19422058 doi:10.1002/prot.22432
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