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| | ==Structure of the Connexin40 Carboxyl terminal Domain== | | ==Structure of the Connexin40 Carboxyl terminal Domain== |
| - | <StructureSection load='2k7m' size='340' side='right' caption='[[2k7m]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='2k7m' size='340' side='right'caption='[[2k7m]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2k7m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K7M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2K7M FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2k7m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K7M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K7M FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Gja5, Cxn-40 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k7m OCA], [http://pdbe.org/2k7m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2k7m RCSB], [http://www.ebi.ac.uk/pdbsum/2k7m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2k7m ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k7m OCA], [https://pdbe.org/2k7m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k7m RCSB], [https://www.ebi.ac.uk/pdbsum/2k7m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k7m ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CXA5_RAT CXA5_RAT]] One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. | + | [https://www.uniprot.org/uniprot/CXA5_RAT CXA5_RAT] One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | ==See Also== | | ==See Also== |
| - | *[[Connexin|Connexin]] | + | *[[Connexin 3D structure|Connexin 3D structure]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Buffalo rat]] | + | [[Category: Large Structures]] |
| - | [[Category: Bouvier, D]] | + | [[Category: Rattus norvegicus]] |
| - | [[Category: Forge, V]] | + | [[Category: Bouvier D]] |
| - | [[Category: Kellezi, A]] | + | [[Category: Forge V]] |
| - | [[Category: Kieken, F]] | + | [[Category: Kellezi A]] |
| - | [[Category: Spagnol, G]] | + | [[Category: Kieken F]] |
| - | [[Category: Vitrac, H]] | + | [[Category: Spagnol G]] |
| - | [[Category: Cell junction]]
| + | [[Category: Vitrac H]] |
| - | [[Category: Cell membrane]]
| + | |
| - | [[Category: Gap junction]]
| + | |
| - | [[Category: Membrane]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Protein]]
| + | |
| - | [[Category: Transmembrane]]
| + | |
| Structural highlights
Function
CXA5_RAT One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Gap junctions are intercellular channels that allow the passage of ions, small molecules, and second messengers that are essential for the coordination of cellular function. They are formed by two hemichannels, each constituted by the oligomerization of six connexins (Cx). Among the 21 different human Cx isoforms, studies have suggested that in the heart, Cx40 and Cx43 can oligomerize to form heteromeric hemichannels. The mechanism of heteromeric channel regulation has not been clearly defined. Tissue ischemia leads to intracellular acidification and closure of Cx43 and Cx40 homomeric channels. However, coexpression of Cx40 and Cx43 in Xenopus oocytes enhances the pH sensitivity of the channel. This phenomenon requires the carboxyl-terminal (CT) part of both connexins. In this study we used different biophysical methods to determine the structure of the Cx40CT and characterize the Cx40CT/Cx43CT interaction. Our results revealed that the Cx40CT is an intrinsically disordered protein similar to the Cx43CT and that the Cx40CT and Cx43CT can interact. Additionally, we have identified an interaction between the Cx40CT and the cytoplasmic loop of Cx40 as well as between the Cx40CT and the cytoplasmic loop of Cx43 (and vice versa). Our studies support the "particle-receptor" model for pH gating of Cx40 and Cx43 gap junction channels and suggest that interactions between cytoplasmic regulatory domains (both homo- and hetero-connexin) could be important for the regulation of heteromeric channels.
Characterization of the structure and intermolecular interactions between the connexin40 and connexin43 carboxyl-terminal and cytoplasmic loop domains.,Bouvier D, Spagnol G, Chenavas S, Kieken F, Vitrac H, Brownell S, Kellezi A, Forge V, Sorgen PL J Biol Chem. 2009 Dec 4;284(49):34257-71. Epub 2009 Oct 5. PMID:19808665[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Bouvier D, Spagnol G, Chenavas S, Kieken F, Vitrac H, Brownell S, Kellezi A, Forge V, Sorgen PL. Characterization of the structure and intermolecular interactions between the connexin40 and connexin43 carboxyl-terminal and cytoplasmic loop domains. J Biol Chem. 2009 Dec 4;284(49):34257-71. Epub 2009 Oct 5. PMID:19808665 doi:10.1074/jbc.M109.039594
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