2ke8

<StructureSection load='2ke8' size='340' side='right' caption='[[2ke8]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>

<table><tr><td colspan='2'>[[2ke8]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KE8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KE8 FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AG:SILVER+ION'>AG</scene></td></tr>

<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=D33:1-(2-DEOXY-5-O-PHOSPHONO-BETA-D-ERYTHRO-PENTOFURANOSYL)-1H-IMIDAZOLE'>D33</scene></td></tr>

<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2k67|2k67]], [[2k68|2k68]], [[2k69|2k69]]</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ke8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ke8 OCA], [http://pdbe.org/2ke8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ke8 RCSB], [http://www.ebi.ac.uk/pdbsum/2ke8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ke8 ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Metal-mediated base pairs represent a powerful tool for the site-specific functionalization of nucleic acids with metal ions. The development of applications of the metal-modified nucleic acids will depend on the availability of structural information on these double helices. We present here the NMR solution structure of a self-complementary DNA oligonucleotide with three consecutive imidazole nucleotides in its centre. In the absence of transition-metal ions, a hairpin structure is adopted with the artificial nucleotides forming the loop. In the presence of Ag(i) ions, a duplex comprising three imidazole-Ag(+)-imidazole base pairs is formed. Direct proof for the formation of metal-mediated base pairs was obtained from (1)J((1)N,(1)/(1)Ag) couplings upon incorporation of (1)N-labelled imidazole. The duplex adopts a B-type conformation with only minor deviations in the region of the artificial bases. This work represents the first structural characterization of a metal-modified nucleic acid with a continuous stretch of metal-mediated base pairs.

Solution structure of a DNA double helix with consecutive metal-mediated base pairs.,Johannsen S, Megger N, Bohme D, Sigel RK, Muller J Nat Chem. 2010 Mar;2(3):229-34. Epub 2010 Jan 17. PMID:21124482<ref>PMID:21124482</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 2ke8" style="background-color:#fffaf0;"></div>

== References ==

<references/>

[[Category: Boehme, D]]

[[Category: Duepre, N]]

[[Category: Johannsen, S]]

[[Category: Mueller, J]]

[[Category: Sigel, R K.O]]

[[Category: Imidazole nucleoside]]