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| | ==Structural requirements for the UBA domain of the mRNA export factor Mex67 to bind its specific targets, the transcription elongation Tho complex component Hpr1 and nucleoporin FxFG repeats== | | ==Structural requirements for the UBA domain of the mRNA export factor Mex67 to bind its specific targets, the transcription elongation Tho complex component Hpr1 and nucleoporin FxFG repeats== |
| - | <StructureSection load='2khh' size='340' side='right' caption='[[2khh]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2khh' size='340' side='right'caption='[[2khh]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2khh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KHH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KHH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2khh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KHH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KHH FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MEX67 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2khh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2khh OCA], [http://pdbe.org/2khh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2khh RCSB], [http://www.ebi.ac.uk/pdbsum/2khh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2khh ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2khh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2khh OCA], [https://pdbe.org/2khh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2khh RCSB], [https://www.ebi.ac.uk/pdbsum/2khh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2khh ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MEX67_YEAST MEX67_YEAST]] Involved in the export of mRNA from the nucleus to the cytoplasm.<ref>PMID:9214641</ref> | + | [https://www.uniprot.org/uniprot/MEX67_YEAST MEX67_YEAST] Involved in the export of mRNA from the nucleus to the cytoplasm.<ref>PMID:9214641</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 18824]] | + | [[Category: Large Structures]] |
| - | [[Category: Brockmann, C]] | + | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: Dargemont, C]] | + | [[Category: Brockmann C]] |
| - | [[Category: Divita, G]] | + | [[Category: Dargemont C]] |
| - | [[Category: Gruessing, F]] | + | [[Category: Divita G]] |
| - | [[Category: Hobeika, M]] | + | [[Category: Gruessing F]] |
| - | [[Category: Neuhaus, D]] | + | [[Category: Hobeika M]] |
| - | [[Category: Stewart, M]] | + | [[Category: Neuhaus D]] |
| - | [[Category: Cytoplasm]]
| + | [[Category: Stewart M]] |
| - | [[Category: Fxfg]]
| + | |
| - | [[Category: Leucine-rich repeat]]
| + | |
| - | [[Category: Mex67]]
| + | |
| - | [[Category: Mrna export]]
| + | |
| - | [[Category: Mrna transport]]
| + | |
| - | [[Category: Nucleus]]
| + | |
| - | [[Category: Transport]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| - | [[Category: Uba]]
| + | |
| Structural highlights
Function
MEX67_YEAST Involved in the export of mRNA from the nucleus to the cytoplasm.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The ubiquitin-associated (UBA) domain of the principal Saccharomyces cerevisiae mRNA nuclear export factor, Mex67, can bind both nuclear pore protein (nucleoporin) FG repeats and Hpr1, a component of the TREX.THO complex that functions to link transcription and export. Using fluorescence resonance energy transfer-based assays, we show here that Hpr1 and the FG repeats interact with overlapping binding sites on the Mex67 UBA domain. We present the solution structure of the Mex67 UBA domain (UBA-Mex67) complexed with a FXFG nucleoporin peptide and define residues engaged in the interaction and those involved in the FXFG-induced conformational change. We show by NMR titration that the binding of Hpr1 produces analogous changes in chemical shifts in similar regions of the UBA domain. Together the data presented here indicate that both Hpr1 and FXFG nucleoporins may bind in a similar way to the UBA-Mex67 domain. However, whereas binding of Hpr1 allows UBA-Mex67 to interact with tetra-ubiquitin, the complex between UBA-Mex67 and FXFG is unable to bind mono- or tetra-ubiquitin, suggesting that both substrate binding and also the nature of the substrate may influence the affinity of the UBA-Mex67 domain for ubiquitin.
Structural requirements for the ubiquitin-associated domain of the mRNA export factor Mex67 to bind its specific targets, the transcription elongation THO complex component Hpr1 and nucleoporin FXFG repeats.,Hobeika M, Brockmann C, Gruessing F, Neuhaus D, Divita G, Stewart M, Dargemont C J Biol Chem. 2009 Jun 26;284(26):17575-83. Epub 2009 Apr 28. PMID:19401465[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Segref A, Sharma K, Doye V, Hellwig A, Huber J, Luhrmann R, Hurt E. Mex67p, a novel factor for nuclear mRNA export, binds to both poly(A)+ RNA and nuclear pores. EMBO J. 1997 Jun 2;16(11):3256-71. PMID:9214641 doi:http://dx.doi.org/10.1093/emboj/16.11.3256
- ↑ Hobeika M, Brockmann C, Gruessing F, Neuhaus D, Divita G, Stewart M, Dargemont C. Structural requirements for the ubiquitin-associated domain of the mRNA export factor Mex67 to bind its specific targets, the transcription elongation THO complex component Hpr1 and nucleoporin FXFG repeats. J Biol Chem. 2009 Jun 26;284(26):17575-83. Epub 2009 Apr 28. PMID:19401465 doi:10.1074/jbc.M109.004374
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