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| | ==Solution structure of stem-loop alpha of the hepatitis B virus post-transcriptional regulatory element== | | ==Solution structure of stem-loop alpha of the hepatitis B virus post-transcriptional regulatory element== |
| - | <StructureSection load='2jym' size='340' side='right' caption='[[2jym]], [[NMR_Ensembles_of_Models | 15 NMR models]]' scene=''> | + | <StructureSection load='2jym' size='340' side='right'caption='[[2jym]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2jym]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JYM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JYM FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2jym]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JYM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JYM FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jym FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jym OCA], [http://pdbe.org/2jym PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jym RCSB], [http://www.ebi.ac.uk/pdbsum/2jym PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jym ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jym FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jym OCA], [https://pdbe.org/2jym PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jym RCSB], [https://www.ebi.ac.uk/pdbsum/2jym PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jym ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Gorlach, M]] | + | [[Category: Large Structures]] |
| - | [[Category: Ohlenschlager, O]] | + | [[Category: Gorlach M]] |
| - | [[Category: Schwalbe, M]]
| + | [[Category: Ohlenschlager O]] |
| - | [[Category: Hepatitis b virus]] | + | [[Category: Schwalbe M]] |
| - | [[Category: Nmr solution structure]] | + | |
| - | [[Category: Post-transcriptional regulatory element]]
| + | |
| - | [[Category: Rna]]
| + | |
| - | [[Category: Stem-loop alpha]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Chronic hepatitis B virus (HBV) infections may lead to severe diseases like liver cirrhosis or hepatocellular carcinoma (HCC). The HBV post-transcriptional regulatory element (HPRE) facilitates the nuclear export of unspliced viral mRNAs, contains a splicing regulatory element and resides in the 3'-region of all viral transcripts. The HPRE consists of three sub-elements alpha (nucleotides 1151-1346), beta1 (nucleotides 1347-1457) and beta2 (nucleotides 1458-1582), which confer together full export competence. Here, we present the NMR solution structure (pdb 2JYM) of the stem-loop alpha (SLalpha, nucleotides 1292-1321) located in the sub-element alpha. The SLalpha contains a CAGGC pentaloop highly conserved in hepatoviruses, which essentially adopts a CUNG-like tetraloop conformation. Furthermore, the SLalpha harbours a single bulged G residue flanked by A-helical regions. The structure is highly suggestive of serving two functions in the context of export of unspliced viral RNA: binding sterile alpha motif (SAM-) domain containing proteins and/or preventing the utilization of a 3'-splice site contained within SLalpha.
Solution structure of stem-loop alpha of the hepatitis B virus post-transcriptional regulatory element.,Schwalbe M, Ohlenschlager O, Marchanka A, Ramachandran R, Hafner S, Heise T, Gorlach M Nucleic Acids Res. 2008 Mar;36(5):1681-9. Epub 2008 Feb 7. PMID:18263618[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Schwalbe M, Ohlenschlager O, Marchanka A, Ramachandran R, Hafner S, Heise T, Gorlach M. Solution structure of stem-loop alpha of the hepatitis B virus post-transcriptional regulatory element. Nucleic Acids Res. 2008 Mar;36(5):1681-9. Epub 2008 Feb 7. PMID:18263618 doi:http://dx.doi.org/gkn006
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