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| | ==NMR structure of the TFIIE-alpha carboxyl terminus== | | ==NMR structure of the TFIIE-alpha carboxyl terminus== |
| - | <StructureSection load='2jtx' size='340' side='right' caption='[[2jtx]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2jtx' size='340' side='right'caption='[[2jtx]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2jtx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JTX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JTX FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2jtx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JTX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JTX FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GTF2E1, TF2E1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jtx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jtx OCA], [http://pdbe.org/2jtx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jtx RCSB], [http://www.ebi.ac.uk/pdbsum/2jtx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jtx ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jtx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jtx OCA], [https://pdbe.org/2jtx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jtx RCSB], [https://www.ebi.ac.uk/pdbsum/2jtx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jtx ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/T2EA_HUMAN T2EA_HUMAN]] Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase. | + | [https://www.uniprot.org/uniprot/T2EA_HUMAN T2EA_HUMAN] Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Lello, P Di]] | + | [[Category: Large Structures]] |
| - | [[Category: Omichinski, J G]] | + | [[Category: Di Lello P]] |
| - | [[Category: Activation domain]] | + | [[Category: Omichinski JG]] |
| - | [[Category: P53]]
| + | |
| - | [[Category: Tfiie]]
| + | |
| - | [[Category: Tfiih]]
| + | |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Transcription regulation]]
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| Structural highlights
Function
T2EA_HUMAN Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The general transcription factor IIH is recruited to the transcription preinitiation complex through an interaction between its p62/Tfb1 subunit and the alpha-subunit of the general transcription factor IIE (TFIIEalpha). We have determined that the acidic carboxyl terminus of TFIIEalpha (TFIIEalpha(336-439)) directly binds the amino-terminal PH domain of p62/Tfb1 with nanomolar affinity. NMR mapping and mutagenesis studies demonstrate that the TFIIEalpha binding site on p62/Tfb1 is identical to the binding site for the second transactivation domain of p53 (p53 TAD2). In addition, we demonstrate that TFIIEalpha(336-439) is capable of competing with p53 for a common binding site on p62/Tfb1 and that TFIIEalpha(336-439) and the diphosphorylated form (pS46/pT55) of p53 TAD2 have similar binding constants. NMR structural studies reveal that TFIIEalpha(336-439) contains a small domain (residues 395-433) folded in a novel betabetaalphaalphaalpha topology. NMR mapping studies demonstrate that two unstructured regions (residues 377-393 and residues 433-439) located on either side of the folded domain appear to be required for TFIIEalpha(336-439) binding to p62/Tfb1 and that these two unstructured regions are held close to each other in three-dimensional space by the novel structured domain. We also demonstrate that, like p53, TFIIEalpha(336-439) can activate transcription in vivo. These results point to an important interplay between the general transcription factor TFIIEalpha and the tumor suppressor protein p53 in regulating transcriptional activation that may be modulated by the phosphorylation status of p53.
p53 and TFIIEalpha share a common binding site on the Tfb1/p62 subunit of TFIIH.,Di Lello P, Miller Jenkins LM, Mas C, Langlois C, Malitskaya E, Fradet-Turcotte A, Archambault J, Legault P, Omichinski JG Proc Natl Acad Sci U S A. 2008 Jan 8;105(1):106-11. Epub 2007 Dec 26. PMID:18160537[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Di Lello P, Miller Jenkins LM, Mas C, Langlois C, Malitskaya E, Fradet-Turcotte A, Archambault J, Legault P, Omichinski JG. p53 and TFIIEalpha share a common binding site on the Tfb1/p62 subunit of TFIIH. Proc Natl Acad Sci U S A. 2008 Jan 8;105(1):106-11. Epub 2007 Dec 26. PMID:18160537
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