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5z5e

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'''Unreleased structure'''
 
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The entry 5z5e is ON HOLD until Paper Publication
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==Crystal structure of the Glycyl-tRNA synthetase (GlyRS) in Nanoarchaeum equitans==
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<StructureSection load='5z5e' size='340' side='right'caption='[[5z5e]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5z5e]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nanoarchaeum_equitans_Kin4-M Nanoarchaeum equitans Kin4-M]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z5E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Z5E FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.098&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5z5e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z5e OCA], [https://pdbe.org/5z5e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5z5e RCSB], [https://www.ebi.ac.uk/pdbsum/5z5e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5z5e ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q74N31_NANEQ Q74N31_NANEQ]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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This study reports the X-ray crystallographic structure of the glycyl-tRNA synthetase (GlyRS) of Nanoarchaeum equitans - a hyperthermophilic archaeal species. This is the first archaeal GlyRS crystal structure elucidated. The GlyRS comprises an N-terminal catalytic domain and a C-terminal anticodon-binding domain with a long beta-sheet inserted between these domains. An unmodified transcript of the wild-type N. equitans tRNA(Gly) was successfully glycylated using GlyRS. Substitution of the discriminator base A73 of tRNA(Gly) with any other nucleotide caused a significant decrease in glycylation activity. Mutational analysis of the second base-pair C2G71 of the acceptor stem of tRNA(Gly) elucidated the importance of the base-pair, especially G71, as an identity element for recognition by GlyRS. Glycylation assays using tRNA(Gly) G71 substitution mutants and a GlyRS mutant where Arg223 is mutated to alanine strengthen the possibility that the carbonyl oxygen at position 6 of G71 would hydrogen-bond with the guanidine nitrogen of Arg223 in N. equitans GlyRS.
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Authors: Noguchi, H., Park, S.Y., Tamura, K.
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Glycyl-tRNA synthetase from Nanoarchaeum equitans: The first crystal structure of archaeal GlyRS and analysis of its tRNA glycylation.,Fujisawa A, Toki R, Miyake H, Shoji T, Doi H, Hayashi H, Hanabusa R, Mutsuro-Aoki H, Umehara T, Ando T, Noguchi H, Voet A, Park SY, Tamura K Biochem Biophys Res Commun. 2019 Apr 2;511(2):228-233. doi:, 10.1016/j.bbrc.2019.01.142. Epub 2019 Feb 13. PMID:30771900<ref>PMID:30771900</ref>
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Description: Crystal structure of the Glycyl-tRNA synthetase (GlyRS) in Nanoarchaeum equitans
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Noguchi, H]]
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<div class="pdbe-citations 5z5e" style="background-color:#fffaf0;"></div>
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[[Category: Tamura, K]]
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== References ==
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[[Category: Park, S.Y]]
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Nanoarchaeum equitans Kin4-M]]
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[[Category: Noguchi H]]
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[[Category: Park SY]]
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[[Category: Tamura K]]

Current revision

Crystal structure of the Glycyl-tRNA synthetase (GlyRS) in Nanoarchaeum equitans

PDB ID 5z5e

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