2bhf

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[[Image:2bhf.gif|left|200px]]<br />
 
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<applet load="2bhf" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2bhf, resolution 2.50&Aring;" />
 
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'''3D STRUCTURE OF THE REDUCED FORM OF COTA'''<br />
 
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==Overview==
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==3D structure of the reduced form of CotA==
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The multi-copper oxidases oxidise substrate molecules by accepting, electrons at a mononuclear copper centre and transferring them to a, trinuclear centre. Dioxygen binds to the trinuclear centre and, following, the transfer of four electrons, is reduced to two molecules of water. The, precise mechanism of this reduction has been unclear, but recent X-ray, structural studies using the CotA endospore coat protein from Bacillus, subtilis have given further insights into the principal stages. It is, proposed that the mechanism involves binding of the dioxygen into the, trinuclear centre so that it is sited approximately symmetrically between, the two type 3 copper ions with one oxygen atom close to the type 2 copper, ion. Further stages involve the formation of a peroxide intermediate and, following the splitting of this intermediate, the migration of the, hydroxide moieties towards the solvent exit channel. The migration steps, are likely to involve a movement of the type 2 copper ion and its, environment. Details of a putative mechanism are described herein based, both on structures already reported in the literature and on structures of, the CotA protein in the oxidised and reduced states and with the addition, of peroxide and the inhibitor, azide.
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<StructureSection load='2bhf' size='340' side='right'caption='[[2bhf]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2bhf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BHF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BHF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bhf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bhf OCA], [https://pdbe.org/2bhf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bhf RCSB], [https://www.ebi.ac.uk/pdbsum/2bhf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bhf ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/COTA_BACSU COTA_BACSU] Involved in brown pigmentation during sporogenesis.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bh/2bhf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bhf ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The multi-copper oxidases oxidise substrate molecules by accepting electrons at a mononuclear copper centre and transferring them to a trinuclear centre. Dioxygen binds to the trinuclear centre and, following the transfer of four electrons, is reduced to two molecules of water. The precise mechanism of this reduction has been unclear, but recent X-ray structural studies using the CotA endospore coat protein from Bacillus subtilis have given further insights into the principal stages. It is proposed that the mechanism involves binding of the dioxygen into the trinuclear centre so that it is sited approximately symmetrically between the two type 3 copper ions with one oxygen atom close to the type 2 copper ion. Further stages involve the formation of a peroxide intermediate and following the splitting of this intermediate, the migration of the hydroxide moieties towards the solvent exit channel. The migration steps are likely to involve a movement of the type 2 copper ion and its environment. Details of a putative mechanism are described herein based both on structures already reported in the literature and on structures of the CotA protein in the oxidised and reduced states and with the addition of peroxide and the inhibitor, azide.
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==About this Structure==
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Dioxygen reduction by multi-copper oxidases; a structural perspective.,Bento I, Martins LO, Gato Lopes G, Armenia Carrondo M, Lindley PF Dalton Trans. 2005 Nov 7;(21):3507-13. Epub 2005 Sep 27. PMID:16234932<ref>PMID:16234932</ref>
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2BHF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with CU1 and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BHF OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Dioxygen reduction by multi-copper oxidases; a structural perspective., Bento I, Martins LO, Gato Lopes G, Armenia Carrondo M, Lindley PF, Dalton Trans. 2005 Nov 7;(21):3507-13. Epub 2005 Sep 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16234932 16234932]
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</div>
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[[Category: Bacillus subtilis]]
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<div class="pdbe-citations 2bhf" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Bento, I.]]
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[[Category: Carrondo, M.A.]]
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[[Category: Lindley, P.F.]]
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[[Category: Lopes, G.G.]]
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[[Category: Martins, L.O.]]
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[[Category: CU1]]
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[[Category: GOL]]
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[[Category: laccase]]
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[[Category: multicopper-oxidase]]
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[[Category: oxidoreductase]]
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[[Category: oxygen reduction]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 17:38:45 2007''
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==See Also==
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*[[Laccase 3D structures|Laccase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Bacillus subtilis]]
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[[Category: Large Structures]]
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[[Category: Bento I]]
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[[Category: Carrondo MA]]
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[[Category: Lindley PF]]
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[[Category: Lopes GG]]
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[[Category: Martins LO]]

Current revision

3D structure of the reduced form of CotA

PDB ID 2bhf

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